Multicenter Evaluation of a New Shortened Peptide Nucleic Acid Fluorescence In Situ Hybridization Procedure for Species Identification of Select Gram-Negative Bacilli from Blood Cultures

• Published in: Journal of Clinical Microbiology Vol. 48; no. 6; pp. 2268 - 2270
• Main Authors: Morgan, Margie, Marlowe, Elizabeth, Della-Latta, Phyllis, Salimnia, Hossein, Novak-Weekley, Susan, Wu, Fann, Crystal, Benjamin S
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.06.2010; American Society for Microbiology (ASM)
• Subjects: Bacteriological Techniques - methods; Bacteriology; Biological and medical sciences; Blood - microbiology; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - isolation & purification; Gram-Negative Bacterial Infections - diagnosis; Gram-Negative Bacterial Infections - microbiology; Humans; In Situ Hybridization, Fluorescence - methods; Microbiology; Peptide Nucleic Acids; Sensitivity and Specificity; Identification; Peptides; Blood culture; Nucleic acid; Fluorescence in situ hybridization; Gram negative bacteria
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.00166-10

A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.