Distribution and accumulation of storage protein-1 in ovary of Hyphantria cunea Drury

• Published in: Archives of insect biochemistry and physiology Vol. 37; no. 2; pp. 115 - 128
• Main Authors: Seo, S.J. (Gyeongsang National University, Gyeongnam, Korea.), Kang, Y.J, Cheon, H.M, Kim, H.R
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 1998
• Subjects: ABSORCION DIGESTIVA; ABSORPTION DIGESTIVE; ACIDE AMINE; ADULTE; ADULTOS; ADULTS; AMINO ACIDS; Amino Acids - analysis; AMINOACIDOS; ANIMAL PROTEINS; Animals; APPAREIL DIGESTIF; DIGESTIVE ABSORPTION; DIGESTIVE SYSTEM; FAT BODY; Female; HAEMOLYMPH; HEMOLINFA; HEMOLYMPHE; HYPHANTRIA CUNEA; immunogold labeling; Immunohistochemistry; Insect Proteins - chemistry; Insect Proteins - metabolism; LOCALIZATION; METHIONINE; METHIONINE RICH STORAGE PROTEIN; METIONINA; Moths - metabolism; oocyte; OOCYTES; Oocytes - metabolism; OOGENESIS; OVA; OVAIRE; OVARIAN DEVELOPMENT; OVARIES; OVARIOS; Ovary - metabolism; OVOGENESE; OVULE; OVULO; PROTEIN UPTAKE; PROTEINAS; PROTEINE; PROTEINS; PUPAE; PUPAS; PUPE; SISTEMA DIGESTIVO; VITELLOGENESIS; YOLK BODY
• ISSN: 0739-4462; 1520-6327
• DOI: 10.1002/(SICI)1520-6327(1998)37:2<115::AID-ARCH1>3.0.CO;2-T

Storage protein-1 (SP-1) is a major storage protein found in the hemolymph and fat body of Hyphantria cunea. In this study, the uptake and accumulation of SP-1 into the ovary of H. cunea was investigated using biochemical and immunocytochemical methods. SP-1 in H. cunea has a high methionine content (4.6%) but is not female-specific, like other high methionine storage proteins. In the 6-day-old pupal ovary, SP-1 was detectable in trace amounts but accumulated to significant levels toward the end of the pupal stage. After adult emergence, SP-1 rapidly decreased in the ovarian follicles and remained low in the egg. This suggest that SP-1 is either extensively modified or degraded, causing a loss of its antigenic property in the ovary after adult emergence. During vitellogenesis, SP-1 is present in the hemolymph and penetrates through the tunica propria to reach the perioocytic space. From there, SP-1 is incorporated into yolk bodies. These results clearly show that SP-1 is taken up by the developing oocyte. Its disappearance suggests that SP-1 might be an amino acid reservoir for providing precursors for egg formation, in contrast to yolk proteins, which are utilized during postembryonic development