Filamentous vibriophage fs2 encoding the rstC gene integrates into the same chromosomal region as cholera toxin X

• Published in: FEMS microbiology letters Vol. 284; no. 2; pp. 225 - 230
• Main Authors: Nguyen, Dong Tu, Nguyen, Binh Minh, Tran, Huy Hoang, Ngo, Tuan Cuong, Le, Thanh Huong, Nguyen, Hoai Thu, Albert, M. John, Iwami, Mamoru, Ehara, Masahiko
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.07.2008; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Bacteria; Biological and medical sciences; Cholera; Cholera toxin; Chromosome 1; Chromosomes; filamentous phage fs2; Fundamental and applied biological sciences. Psychology; Gene expression; Gene sequencing; Genomes; Homology; Microbiology; Molecular and cellular biology; Molecular genetics; Pathogens; Phages; rstC; site-specific integration; Toxins; Vibrio cholerae O1 and O139; Waterborne diseases; C; site-specific integration; O1 and O139; filamentous phage fs2; RNA; rstC; Vibrio cholerae; Electron microscopy; Virus; Infection; Polymerase chain reaction; Toxin; Gene; DNA; Bacteriosis; Bacteria; Vibrionaceae; Cholera; Vibrio cholerae 01 and 0139; Phage
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2008.01200.x

The genome of the filamentous phage of Vibrio cholerae fs2 was found to contain rstC and rstB1 (truncated) genes downstream of ORF500. att-fs2-dir and att-fs2-rev sequences homologous to that of att-CTXφ were found between orf500 and rstC of the fs2 genome. This prompted us to search for the integration site of fs2 in the genomes of V. cholerae O1 and O139. The genome of fs2 was found to integrate downstream of attRS of the CTXφ phage, which integrated into chromosome I of V. cholerae O1 and O139. When infected with fs2, a fimbriate strain of V. cholerae O1 appeared to reduce fimbrial production in an adult rabbit ileal loop assay.