Circadian-regulated transcription of the psbD light-responsive promoter in wheat chloroplasts

• Published in: Plant physiology (Bethesda) Vol. 118; no. 3; pp. 1079 - 1088
• Main Authors: Nakahira, Y. (Kyoto University, Kyoto, Japan.), Baba, K, Yoneda, A, Shiina, T, Toyoshima, Y
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.11.1998
• Subjects: ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Biological and medical sciences; BIOLOGICAL RHYTHMS; CHLOROPLASTE; CHLOROPLASTS; Chloroplasts - metabolism; CIRCADIAN RHYTHM; CLOROPLASTO; DARKNESS; DNA probes; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Gene Expression Regulation, Plant; Genes; GENETIC VARIATION; LIGHT; LUMIERE; LUZ; MESSENGER RNA; Molecular and cellular biology; Molecular genetics; Molecular Genetics and Genomics; MOLECULAR SEQUENCE DATA; MUTANT; MUTANTES; MUTANTS; NUCLEOTIDE SEQUENCE; OBSCURIDAD; OBSCURITE; Photosynthetic Reaction Center Complex Proteins - genetics; Photosystem II Protein Complex; Plants; Plastids; Plastids - metabolism; Promoter Regions, Genetic; QUANTITATIVE ANALYSIS; RITMOS BIOLOGICOS; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; RYTHME BIOLOGIQUE; SECUENCIA NUCLEOTIDICA; Seedlings; Sequence Homology, Nucleic Acid; SEQUENCE NUCLEOTIDIQUE; TRANSCRIPCION; TRANSCRIPTION; Transcription initiation site; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Transcriptional regulatory elements; Triticum - genetics; Triticum - metabolism; TRITICUM AESTIVUM; VARIACION GENETICA; VARIATION GENETIQUE; Monocotyledones; Transcription; Transcription promoter; Site directed mutagenesis; Environmental factor; Circadian rhythm; Cereal crop; Regulation(control); Messenger RNA; Structure activity relation; Gramineae; Light; Angiospermae; Spermatophyta; Chloroplast; Photoregulation; Triticum aestivum
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.118.3.1079

The level of mRNAs derived from the plastid-coded psbD light-responsive promoter (LRP) is controlled by a circadian clock(s) in wheat (Triticum aestivum). The circadian oscillations in the LRP mRNA level persisted for at least three cycles in continuous light and for one cycle in continuous dark, with maxima in subjective morning and minima in subjective early night. In vitro transcription in chloroplast extracts revealed that the circadian cycles in the psbD LRP mRNA level were dominantly attributed to the circadian-regulated transcription of the psbD LRP. The effects of various mutations introduced into the promoter region on the psbD LRP activity in vitro suggest the existence of two positive elements located between -54 and -36, which generally enhance the transcription activity, and an anomalous core promoter structure lacking the functional "-35" element, which plays a crucial role in the circadian fluctuation and light dependency of psbD LRP transcription activity