Polyphenol oxidase in potato. A multigene family that exhibits differential expression patterns

• Published in: Plant physiology (Bethesda) Vol. 109; no. 2; pp. 525 - 531
• Main Authors: Peter W. Thygesen, Dry, Ian B., Simon P. Robinson
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.10.1995
• Subjects: actividad enzimatica; activite enzymatique; adn; Amino Acid Sequence; arn mensajero; arn messager; Biological and medical sciences; catechol oxidase; Catechol Oxidase - biosynthesis; Catechol Oxidase - genetics; Catechol Oxidase - metabolism; catechol oxydase; catecol oxidasa; chemical composition; Cloning, Molecular; Complementary DNA; composicion quimica; composition chimique; dna; DNA, Complementary; DNA, Plant - genetics; DNA, Plant - isolation & purification; Enzymes; enzymic activity; etapas de desarrollo de la planta; expresion genica; expression des genes; Flower buds; Fundamental and applied biological sciences. Psychology; gene; Gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Gene Regulation and Molecular Genetics; genes; Genes, Plant; Kinetics; Leaves; messenger rna; Metabolism; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; nucleotide sequence; Oxidases; Petals; plant developmental stages; Plant physiology and development; Plant Roots; plant tissues; Plants; Polyphenols; Proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - metabolism; RNA; secuencia nucleotidica; Sequence Homology, Amino Acid; sequence nucleotidique; solanum tuberosum; Solanum tuberosum - enzymology; Solanum tuberosum - genetics; Solanum tuberosum - growth & development; stade de developpement vegetal; tejidos vegetales; tissu vegetal; tubercule; tuberculo; Tubers; Enzyme; Solanum tuberosum; Catechol oxidase; Homology; Gene expression; Characterization; Enzymatic activity; Gene; Dicotyledones; Angiospermae; Tuber; Multigene family; Spermatophyta; Developmental stage; Oxidoreductases; Tuber plant; Solanaceae; Aminoacid sequence
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.109.2.525

Polyphenol oxidase (PPO) activity in potato (Solanum tuberosum) plants was high in stolons, tubers, roots, and flowers but low in leaves and stems. PPO activity per tuber continued to increase throughout tuber development but was highest on a fresh weight basis in developing tubers. PPO activity was greatest at the tuber exterior, including the skin and cortex tissue 1 to 2 mm beneath the skin. Flowers had high PPO activity throughout development, particularly in the anthers and ovary. Five distinct cDNA clones encoding PPO were isolated from developing tuber RNA. POT32 was the major form expressed in tubers and was found in all parts of the tuber and at all stages of tuber development. It was also expressed in roots but not in photosynthetic tissues. POT33 was expressed in tubers but mainly in the tissue near the skin. POT72 was detected in roots and at low levels in developing tubers. NOR333 was identical with the P2 PPO clone previously isolated from potato leaves (M.D. Hunt, N.T. Eannetta, Y. Haifeng, S.M. Newman, I.C. Steffens [1993] Plant Mol Biol 21: 59-68) and was detected in young leaves and in tissue near the tuber skin but was highly expressed in flowers. The results indicate that PPO is present as a small multigene family in potato and that each gene has a specific temporal and spatial pattern of expression.