Genotypic and Phenotypic Evidence of Different Drug-Resistance Mutation Patterns between B and Non-B Subtype Isolates of Human Immunodeficiency Virus Type 1 found in Brazilian Patients Failing HAART

• Published in: Virus genes Vol. 23; no. 2; pp. 193 - 202
• Main Authors: Caride, E, Hertogs, K, Larder, B, Dehertogh, P, Brindeiro, R, Machado, E, de Sá, C A, Eyer-Silva, W A, Sion, F S, Passioni, L F, Menezes, J A, Calazans, A R, Tanuri, A
• Format: Journal Article
• Language: English
• Published: United States Kluwer Academic Publishers 01.01.2001; Springer Nature B.V
• Subjects: Amino Acid Sequence; antiretroviral therapy; Antiretroviral Therapy, Highly Active; Drug Resistance, Microbial - genetics; Genotype; highly active antiretroviral therapy; HIV Infections - drug therapy; HIV Infections - virology; HIV-1 - drug effects; HIV-1 - genetics; Human immunodeficiency virus 1; Humans; Molecular Sequence Data; Mutation; Phenotype; pol gene; Sequence Homology, Amino Acid; Brazil
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1023/A:1011812810397

We have investigated the phenotypic and genotypic susceptibility of 14 HIV-1 strains isolated from individuals failing HAART therapy to protease inhibitors (PI). Proviral and plasma viral pol gene fragment were amplified, sequenced and subtyped. Nine samples clustered with protease subtype B reference strains and the remaining samples were classified as non-B subtype corresponding to subtype F (n=4) and subtype A (n=1). Although all patients were treated with similar PI drug regimen, the non-B subtype isolates did not present the L90M and I84V mutations and used mainly G48V and V82A/F to achieve drug resistance. A strong cross-resistance phenotype among all four PI was associated with the mutation L90M in the subtype-B isolates, and with G48V and V82A/F in the non-B counterparts. This observation revealed that the non-B viruses tested had specific genotypic characteristics contrasting with the subtype-B isolates.