Mutants of the phytopathogenic fungus Magnaporthe grisea deficient in alternative, cyanide-resistant, respiration

The phytopathogenic fungus Magnaporthe grisea has a cyanide-resistant respiratory pathway. The fungicide SSF-126 ((E)-2-methoxyimino-N-methyl-2-(2-phenoxyphenyl) acetamide) blocks the cytochrome electron transport of M. grisea and induces the alternative respiratory pathway. Twelve mutants of M. gri...

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Published inFungal genetics and biology Vol. 22; no. 3; pp. 221 - 228
Main Authors Yukioka, H, Tanaka, R, Inagaki, S, Katoh, K, Miki, N, Mizutani, A, Masuko, M, Kunoh, H
Format Journal Article
LanguageEnglish
Published United States 1997
Subjects
alternative respiration
electron transfer
electron transport chain
fungicides
immunoblotting
immunochemistry
Magnaporthe
Magnaporthe grisea
mutagenesis
mutants
oxidoreductases
respiration
ssf-126
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Summary:The phytopathogenic fungus Magnaporthe grisea has a cyanide-resistant respiratory pathway. The fungicide SSF-126 ((E)-2-methoxyimino-N-methyl-2-(2-phenoxyphenyl) acetamide) blocks the cytochrome electron transport of M. grisea and induces the alternative respiratory pathway. Twelve mutants of M. grisea more susceptible to SSF-126 than wild type were identified after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. Five mutants retained a reduced alternative respiration activity, and seven mutants lacked alternative pathway activity. A monoclonal antibody against the maize alternative oxidase cross-reacted against a 40-kDa mitochondrial protein of M. grisea, indicating that the 40-kDa protein is an alternative oxidase. Immunoblot analysis indicated that the seven completely deficient mutants grouped into two classes: four mutants produced the 40-kDa proteins while the other three mutants failed to produce the functional protein. Copyright 1997 Academic Press. Copyright 1997 Academic Press
ISSN:1087-1845
1096-0937
DOI:10.1006/fgbi.1997.1016