Production of beta-xylosidase activity by Trichoderma harzianum strains

Nine Trichoderma harzianum strains were screened for beta-xylosidase activity when grown in solid-state cultures on media containing wheat bran as the carbon source. All strains produced beta-xylosidase activity, the most active being in extracts of cultures of T. harzianum strain 4. A beta-xylosida...

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Bibliographic Details
Published inCurrent microbiology Vol. 33; no. 2; p. 71
Main Authors de A Ximenes, F, de Paula Silveira, F Q, Filho, E X
Format Journal Article
LanguageEnglish
Published United States 01.08.1996
Subjects
actividad enzimatica
activite enzymatique
biosintesis
biosynthese
biosynthesis
chemicophysical properties
enzymic activity
Molecular Weight
propiedades fisico quimicas
propriete physicochimique
purificacion
purification
Species Specificity
Trichoderma - enzymology
trichoderma harzianum
Xylosidases - isolation & purification
Xylosidases - metabolism
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Summary:Nine Trichoderma harzianum strains were screened for beta-xylosidase activity when grown in solid-state cultures on media containing wheat bran as the carbon source. All strains produced beta-xylosidase activity, the most active being in extracts of cultures of T. harzianum strain 4. A beta-xylosidase was purified by ammonium sulfate precipitation, ultrafiltration, gel filtration, and ion exchange chromatography from solid-state cultures of T. harzianum strain C. Enzyme preparations yielded a single band when stained for protein following electrophoresis. The molecular weight value, calculated following SDS-PAGE, was determined to be 60 kDa. beta-Xylosidase was most active at pH 4.0-4.5 and 70 degrees C. This enzyme had a Km value of 0.053 mM. The phenol-sulfuric acid method detected the presence of a small amount of carbohydrate in the purified enzyme preparation. beta-Xylosidase was active against some p-nitrophenylglycosides. The enzyme was inactive against xylan and PNPG. beta-xylosidase activity was inhibited by xylose and SDS. Iodoacetamide, dithiothreitol, gluconolactone, glucose, and mercuric chloride failed to inactivate this enzyme's activity. A synergistic effect was observed when beta-xylosidase from T. harzianum strain C and beta-xylanase from Aspergillus fumigatus were incubated with pretreated arabinoxylan.
Bibliography:F60
F
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900077