Ratiometric Fluorescent Probe Based on FRET for Imaging Hg²⁺ Ions in Living Cells

Mercury rising: A FRET‐based ratiometric probe (see picture; FRET=fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts‐per‐billion scale under physiological conditions. The probe undergoes a clear Hg2+‐induced change in the intensity ratio of the two well‐separated a...

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Published inAngewandte Chemie (International ed.) Vol. 47; no. 42; pp. 8025 - 8029
Main Authors Zhang, Xiaolin, Xiao, Yi, Qian, Xuhong
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 01.01.2008
WILEY-VCH Verlag
WILEY‐VCH Verlag
Wiley
Subjects
Cell Line, Tumor
Cell Survival
Chemistry
Chemistry, Multidisciplinary
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
fluorescent probes
FRET
Humans
Ions - analysis
Ions - chemistry
mercury
Mercury - analysis
Mercury - chemistry
Mercury - metabolism
Molecular Structure
Physical Sciences
rhodamine
Science & Technology
sensors
DESIGN
RECOGNITION
mercury
SENSOR
fluorescent probes
RESONANCE-ENERGY-TRANSFER
TURN-ON
CHEMOSENSOR
sensors
HG(II)
FRET
MERCURIC ION
rhodamine
WATER
CHEMODOSIMETER
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Summary:Mercury rising: A FRET‐based ratiometric probe (see picture; FRET=fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts‐per‐billion scale under physiological conditions. The probe undergoes a clear Hg2+‐induced change in the intensity ratio of the two well‐separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells.
Bibliography:http://dx.doi.org/10.1002/anie.200803246
Program for Changjiang Scholars and Innovative Research Team in University - No. IRT0711
istex:F0A4CFE6D2D4B91E2E6628D3E6AC9C01D9F74E8D
NSF of China - No. 20406004; No. 20572012; No. 20536010
ArticleID:ANIE200803246
We thank the NSF of China (Nos. 20406004, 20572012, 20536010) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT0711) for financial support. We also thank Dr. Liji Jin and Xueliang Niu (Dalian University of Technology) for help with cell culture experiments.
ark:/67375/WNG-XXRT3S5W-3
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200803246