Acetate and arachidonate incorporations into lipids of ovine chorioallantoic tissue at day 24 of pregnancy

• Published in: Biology of reproduction Vol. 40; no. 4; pp. 785 - 791
• Main Authors: Waterman, R.A. (Beltsville Agricultural Research Center, ARS, USDA, Beltsville, MD), Lewis, G.S
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01.04.1989
• Subjects: ACETATE; ACETATES; Acetates - metabolism; ACETATOS; Acetic Acid; ACIDE GRAS; ACIDOS GRASOS; Allantois - metabolism; Animals; Arachidonic Acid; Arachidonic Acids - metabolism; Biological and medical sciences; BREBIS; Cholesterol Esters - metabolism; Chorion - metabolism; Esterification; EWES; Extraembryonic Membranes - metabolism; FATTY ACIDS; Fatty Acids - metabolism; Female; FETO; FOETUS; Fundamental and applied biological sciences. Psychology; GESTACION; GESTATION; Hormone metabolism and regulation; Kinetics; Lipid Metabolism; LIPIDE; LIPIDOS; LIPIDS; Lipoxygenase - metabolism; OVEJA; Phospholipids - metabolism; PREGNANCY; Pregnancy, Animal - metabolism; Pregnancy. Parturition. Lactation; Prostaglandin-Endoperoxide Synthases - metabolism; Sheep; Triglycerides - metabolism; Vertebrates: reproduction; Pregnancy; Vertebrata; Reproduction; Mammalia; Chorioallantoic membrane; Sheep; Artiodactyla; Acetate; Arachidonic acid; Metabolism; Fatty acids; Ungulata

Incorporation of acetate and arachidonic acid into lipid classes was examined in chorioallantoic membranes obtained from sheep at Day 24 of pregnancy. Conceptus tissues were incubated in vitro with 5 mM acetate, 0.042 mM arachidonate, 0.45 muCi [1-14C]acetate, and 5.0 muCi [5,6,8,9,11,12,14,15-3H]ar...