Acetate and arachidonate incorporations into lipids of ovine chorioallantoic tissue at day 24 of pregnancy
Incorporation of acetate and arachidonic acid into lipid classes was examined in chorioallantoic membranes obtained from sheep at Day 24 of pregnancy. Conceptus tissues were incubated in vitro with 5 mM acetate, 0.042 mM arachidonate, 0.45 muCi [1-14C]acetate, and 5.0 muCi [5,6,8,9,11,12,14,15-3H]ar...
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Published in | Biology of reproduction Vol. 40; no. 4; pp. 785 - 791 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Society for the Study of Reproduction
01.04.1989
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Subjects | |
Online Access | Get full text |
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Summary: | Incorporation of acetate and arachidonic acid into lipid classes was examined in chorioallantoic membranes obtained from sheep
at Day 24 of pregnancy. Conceptus tissues were incubated in vitro with 5 mM acetate, 0.042 mM arachidonate, 0.45 muCi [1-14C]acetate,
and 5.0 muCi [5,6,8,9,11,12,14,15-3H]arachidonate for 3 and 6 h. After incubation, tissue lipid fractions were extracted,
isolated, and examined for radiolabel incorporations. Medium was extracted and analyzed for radiolabeled metabolites. Metabolic
pathways commonly associated with fatty acid metabolism were confirmed to be present. Acetate was utilized for de novo synthesis
of free cholesterol and free fatty acid. Fatty acids containing radiolabel from both acetate and arachidonate were mainly
esterified in phospholipid and triglyceride, major lipid classes found in chorioallantoic tissue. Labeled metabolites of acetate
were not sufficient for analytical measurement in medium. Metabolites of arachidonic acid from lipoxygenase and cyclooxygenase
pathways were determined in medium after incubation. Results suggest that, within Day 24 ovine chorioallantoic tissue, utilization
of exogenous arachidonate and de novo lipogenesis from acetate function in a parallel and anabolic mode appropriate for membrane
expansion. |
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Bibliography: | 9011327 L53 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod40.4.785 |