Vitamins Q and E, extracorporal circulation and hemolysis

• Published in: Molecular and cellular biochemistry Vol. 173; no. 1/2; pp. 33 - 41
• Main Authors: Karlsson, J, Ronneberg, R, Semb, B
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.08.1997
• Subjects: Aged; Antioxidants; Blood; Cholesterol - blood; diet-related diseases; Extracorporeal Circulation; Hemodilution; Hemolysis - physiology; human nutrition; Humans; Male; Medicin och hälsovetenskap; Middle Aged; Myocardial Ischemia - physiopathology; Myocardial Ischemia - surgery; Organic Chemicals; Reference Values; Thoracic surgery; Ubiquinone; Vitamin E - blood; Vitamins; Vitamins - blood
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1023/A:1006874923181

Whole blood vitamin Q (ubiquinone), plasma vitamins Q and E (alpha-(alpha-)tocopherol) and free cholesterol (FC) were studied before (control or base-line value, sample I) and during open chest surgery and extracorporal circulation (samples II-IV) in 10 male IHD patients. Identity existed between control whole blood and plasma ubiquinone. During surgery an increased discrepancy with lower plasma vitamin Q levels were seen. Control plasma vitamins Q, E and FC averaged 0.88 +/- 0.16 (SE), 12.1 +/- 2.2 mg X l-1 and 0.75 +/- 0.15 g X l-1. Corresponding molar values were 1.02 +/- 0.17, 28.1 +/- 5.1 micromol X l-1 and 1.94 +/- 0.74 mmol X l-1. Vitamin Q and E decreased continuously and averaged 0.64 mg X l-1 in sample IV (0.74 micromol X l-1, p < 0.001) and 9.4 mg X l-1 in sample III (21.8 micromol X l-1, p < 0.001). Hemolysis in all sample IV vials, ruined all vitamin E determinations. When normalized for FC (NQ and NE), decreases were found to be 17 (IV) and 12% (III), respectively. Large interindividual variations existed. High control NQ and NE values allowed a larger antioxidant vitamin depletion. High NQ seemed also to be a prerequisite for NE depletion. In addition, signs indicated an active liver vitamin Q release for patients rich in control antioxidant values. It was suggested that the antioxidant vitamin depletion did not prevent from radical trauma to membrane structural lipids (especially omega-3 fatty acids or vitamin F1), less membrane fluidity, erythrocyte fragility and hemolysis.