Antimicrobial resistance, virulence determinants and genetic profiles of clinical and nonclinical Enterococcus cecorum from poultry

• Published in: Letters in applied microbiology Vol. 60; no. 2; pp. 111 - 119
• Main Authors: Jackson, C.R, Kariyawasam, S, Borst, L.B, Frye, J.G, Barrett, J.B, Hiott, L.M, Woodley, T.A
• Format: Journal Article
• Language: English
• Published: England Published for the Society for Applied Bacteriology by Blackwell Scientific Publications [c1985-] 01.02.2015; Oxford University Press
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; antibiotic resistance; Antimicrobial agents; antimicrobial resistance; broiler breeders; Chickens - microbiology; Cholera; Drug resistance; Drug Resistance, Bacterial; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Pulsed-Field; Enterococcus - drug effects; Enterococcus - genetics; Enterococcus - isolation & purification; Enterococcus - pathogenicity; Enterococcus cecorum; erythromycin; genes; genetic background; genetic relatedness; genetic relationships; Gram-Positive Bacterial Infections - microbiology; Gram-Positive Bacterial Infections - veterinary; lincomycin; multiple drug resistance; Pathogenesis; Phenotype; Poultry; poultry carcasses; poultry diseases; Poultry Diseases - microbiology; pulsed-field gel electrophoresis; tetracycline; virulence; Virulence - genetics; Virulence Factors - analysis; Virulence Factors - genetics; poultry; virulence; antimicrobial resistance; genetic relatedness; Enterococcus cecorum
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/lam.12374

Enterococcus cecorum has been implicated as a possible cause of disease in poultry. However, the characteristics that contribute to pathogenesis of Ent. cecorum in poultry have not been defined. In this study, Ent. cecorum from carcass rinsates (n = 75) and diseased broilers and broiler breeders (n = 30) were compared based upon antimicrobial resistance phenotype, the presence of virulence determinants and genetic relatedness using pulsed‐field gel electrophoresis (PFGE). Of the 16 antimicrobials tested, Ent. cecorum from carcass rinsates and clinical cases were resistant to ten and six of the antimicrobials, respectively. The majority of Ent. cecorum from carcass rinsates was resistant to lincomycin (54/75; 72%) and tetracycline (46/75; 61·3%) while the highest level of resistance among clinical Ent. cecorum was to tetracycline (22/30; 73·3%) and erythromycin (11/30; 36·7%). Multidrug resistance (resistance to ≥2 antimicrobials) was identified in Ent. cecorum from carcass rinsates (53/75; 70·7%) and diseased poultry (18/30; 60%). Of the virulence determinants tested, efaAfm was present in almost all of the isolates (104/105; 99%). Using PFGE, the majority of clinical isolates clustered together; however, a few clinical isolates grouped with Ent. cecorum from carcass rinsates. These data suggest that distinguishing the two groups of isolates is difficult based upon the characterization criteria used. SIGNIFICANCE AND IMPACT OF THE STUDY: In this study, antimicrobial resistance phenotype, virulence gene profile and genetic relatedness of Enterococcus cecorum isolated from diseased broiler chickens and poultry carcass rinsates were determined. The majority of isolates from both groups were multidrug resistant and harboured few virulence determinants. Results from this study suggest that clinical Ent. cecorum and Ent. cecorum from poultry carcass rinsates may share a common genetic background; clinical Ent. cecorum possess virulence determinants that have not been previously described for this species. Elucidation of those unknown virulence determinants is important for understanding the pathogenesis of Ent. cecorum infections in poultry.