Leaf photosynthesis and respiration of high CO2-grown tobacco plants selected for survival under CO2 compensation point conditions

• Published in: Plant physiology (Bethesda) Vol. 98; no. 3; pp. 949 - 954
• Main Authors: Delgado, E. (Institut d'Estudis Avancats-Universitat de les Illes Balears, Palma de Mallorca, Spain), Azcon-Bieto, J, Aranda, X, Palazon, J, Medrano, H
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.03.1992
• Subjects: Biological and medical sciences; CADENA RESPIRATORIA; Carbon dioxide; CHAINE RESPIRATOIRE; CRECIMIENTO; CROISSANCE; CULTIVO DE CELULAS; CULTURE DE CELLULE; DIOXIDO DE CARBONO; DIOXYDE DE CARBONE; FEUILLE; FOTOSINTESIS; Fundamental and applied biological sciences. Psychology; Haploidy; HOJAS; Leaf area; Leaves; Metabolism; NICOTIANA TABACUM; Nitrogen; PHOTOSYNTHESE; Photosynthesis; Photosynthesis, respiration. Anabolism, catabolism; Plant growth; Plant physiology and development; Plants; Respiration; Sulfur; VARIACION GENETICA; VARIATION GENETIQUE; Carbon dioxide; Stomatal conductance; Genotype; Plant leaf; Nicotiana tabacum; Dicotyledones; Angiospermae; Compensation point; Spermatophyta; Solanaceae; Gas exchange; Photosynthesis; Respiration
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.98.3.949

Four self-pollinated, doubled-haploid tobacco, (Nicotiana tabacum L.) lines (SP422, SP432, SP435, and SP451), selected as haploids by survival in a low CO2 atmosphere, and the parental cv Wisconsin-38 were grown from seed in a growth room kept at high CO2 levels (600-700 parts per million). The selected plants were much larger (especially SP422, SP432, and SP451) than Wisconsin-38 nine weeks after planting. The specific leaf dry weight and the carbon (but not nitrogen and sulfur) content per unit area were also higher in the selected plants. However, the chlorophyll, carotenoid, and alkaloid contents and the chlorophyll a/b ratio varied little. The net CO2 assimilation rate per unit area measured in the growth room at high CO2 was not higher in the selected plants. The CO2 assimilation rate versus intercellular CO2 curve and the CO2 compensation point showed no substantial differences among the different lines, even though these plants were selected for survival under CO2 compensation point conditions. Adult leaf respiration rates were similar when expressed per unit area but were lower in the selected lines when expressed per unit dry weight. Leaf respiration rates were negatively correlated with specific leaf dry weight and with the carbon content per unit area and were positively correlated with nitrogen and sulfur content of the dry matter. The alternative pathway was not involved in respiration in the dark in these leaves. The better carbon economy of tobacco lines selected for low CO2 survival was not apparently related to an improvement of photosynthesis rate but could be related, at least partially, to a significantly reduced respiration (mainly cytochrome pathway) rate per unit carbon