Effects of pH shock on the secretion system in Streptomyces coelicolor A3(2)

• Published in: Journal of microbiology and biotechnology Vol. 18; no. 4; pp. 658 - 662
• Main Authors: Kim, Y.J. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), Song, J.Y. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), Hong, S.K. (Myongji University, Yongin, Republic of Korea), Smith, Colin P. (University of Surrey, Guildford, United Kingdom), Chang, Y.K. (Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea), E-mail: ychang@kaist.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.04.2008; 한국미생물·생명공학회
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Bioreactors; Biotechnology; efflux pumps; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Hydrogen-Ion Concentration; Oligonucleotide Array Sequence Analysis; pH shock; Protein Transport; S. coelicolor; secretion proteins; STREPTOMYCES; Streptomyces coelicolor - genetics; Streptomyces coelicolor - metabolism; Transcription, Genetic; 생물학; Shock; Streptomycetaceae; Streptomyces coelicolor; Secretion; S. coelicolor; secretion proteins; Protein; Actinomycetales; pH; Bacteria; Actinomycetes; efflux pumps; pH shock
• ISSN: 1017-7825

Effects of pH shock on the secretion system of S. coelicolor A3(2) have been investigated at a transcriptional level by using DNA microarrays. Actinorhodin secretion was observed to be highly enhanced when an acidic-pH shock was applied to surface grown cultures of S. coelicolor A3(2). In this culture, a gene of actVA-orf1 encoding a putative efflux pump or transporter protein for actinorhodin was strongly upregulated. A major number of efflux pumps for other metabolites and a major number of secretion proteins for protein secretion were also observed to be upregulated with pH shock. The secretion of actinorhodin was observed to be remarkably enhanced in liquid culture as well.