Nitrospira community composition in nitrifying reactors operated with two different dissolved oxygen levels

• Published in: Journal of microbiology and biotechnology Vol. 18; no. 8; pp. 1470 - 1474
• Main Authors: Park, H.D. (Kolon Engineering and Construction, Yongin, Republic of Korea), E-mail: heedeung@gmail.com, Noguera, Daniel R. (University of Wisconsin, Madison, WI, U.S.A.)
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.08.2008; 한국미생물·생명공학회
• Subjects: Biological and medical sciences; Bioreactors - microbiology; Biotechnology; DISSOLVED OXYGEN; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; NITRIFICACION; NITRIFICATION; nitrite-oxidizing bacteria; Nitrites - metabolism; Nitrosomonadaceae - genetics; Nitrosomonadaceae - metabolism; Nitrosomonadaceae - physiology; Nitrospira; OXIGENO EN DISOLUCION; Oxygen - metabolism; OXYGENE EN DISSOLUTION; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; terminal restriction fragment length polymorphism (t-RFLP); Water Pollutants, Chemical - metabolism; 생물학; Restriction fragment length polymorphism; Nitrites; Nitrospira; nitrite-oxidizing bacteria; Nitrification; terminal restriction fragment length polymorphism (t-RFLP); Bacteria; Oxidation; Dissolved oxygen; Reactor
• ISSN: 1017-7825

Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 (lays. Phylogenetic and terminal restriction fragment length polymorphism analyses based on 16S rRNA gene sequences revealed that the Nitrospira community compositions of the two reactors during the early period related to group 1 and half of the Nitrospira community composition shifted to group 2 in the high-DO reactor after day 179, although there was no significant change in the low-DO reactor. These results suggested that DO was an important factor affecting Nitrospira community compositions in the nitrifying reactors.