Vaccination of shrimp (Penaeus chinensis) against white spot syndrome virus (WSSV)

• Published in: Journal of microbiology and biotechnology Vol. 18; no. 5; pp. 964 - 967
• Main Authors: Ha, Y.M. (Pukyong National University, Busan, Republic of Korea), Gong, S.J. (Pukyong National University, Busan, Republic of Korea), Nguyen, Thi-Hoai (Pukyong National University, Busan, Republic of Korea), Ra, C.H. (Pukyong National University, Busan, Republic of Korea), Kim, K.H. (Pukyong National University, Busan, Republic of Korea), Nam, Y.K. (Pukyong National University, Busan, Republic of Korea), Kim, S.K. (Pukyong National University, Busan, Republic of Korea), E-mail: skkim@pknu.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.05.2008; 한국미생물·생명공학회
• Subjects: Animals; Biological and medical sciences; Biotechnology; Cell Line; Fundamental and applied biological sciences. Psychology; Gene Expression; oral feeding; Penaeidae - immunology; Penaeidae - virology; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - metabolism; shrimp; Spodoptera; VACCIN; Vaccination; VACCINES; VACUNA; Viral Structural Proteins - genetics; Viral Structural Proteins - immunology; Viral Structural Proteins - metabolism; Viral Vaccines - genetics; Viral Vaccines - immunology; Viral Vaccines - metabolism; VP19; VP466; White spot syndrome virus 1 - genetics; White spot syndrome virus 1 - immunology; White spot syndrome virus 1 - metabolism; WSSV; 생물학; White spot syndrome virus; Vaccination; Vaccine; VP19; Macrura; Crustacea; Penaeus chinensis; Arthropoda; WSSV; oral feeding; Decapoda; VP466; Invertebrata; Shrimp
• ISSN: 1017-7825

Two structural protein genes, VP19 and VP466, of white spot syndrome virus (WSSV) were cloned and expressed in Sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against WSSV for shrimps. The cumulative mortalities of the shrimps vaccinated by the injection of rVP19 and rVP466 at 15 days after the challenge with WSSV were 50.2% and 51.8%, respectively. For the vaccination by oral feeding of rVP19 and rVP466, the cumulative mortalities were 49.2% and 89.2%, respectively. These results show that protection against WSSV can be generated in the shrimp, using the viral structural protein as a protein vaccine.