Low Dietary Calcium Levels Modulate Mucosal Caspase Expression and Increase Disease Activity in Mice with Dextran Sulfate Sodium-Induced Colitis

• Published in: The Journal of nutrition Vol. 137; no. 11; pp. 2475 - 2480
• Main Authors: Pele, Laetitia C, Thoree, Vinay, Mustafa, Feras, He, Shijun, Tsaprouni, Loukia, Punchard, Neville A, Thompson, Richard P.H, Evans, Stephen M, Powell, Jonathan J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Nutrition 01.11.2007; American Society for Nutritional Sciences
• Subjects: animal models; Animals; Biological and medical sciences; calcium; Calcium - deficiency; calcium phosphates; Calcium, Dietary - pharmacology; Caspases - metabolism; colitis; Colitis - chemically induced; Colitis - complications; Colitis - enzymology; Colitis - physiopathology; cysteine proteinases; Dextran Sulfate; dietary minerals; Disease Models, Animal; disease severity; enzyme activity; Feeding. Feeding behavior; Female; Fundamental and applied biological sciences. Psychology; histology; Inflammation; Intestinal Mucosa - enzymology; Mice; Mice, Inbred BALB C; nutrient intake; protective effect; receptors; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Calcium; Disease; Nutrition; Enzyme; Cysteine endopeptidases; Mucosa; Rodentia; Caspase; Gene expression; Sulfates; Inorganic element; Micronutrient; Peptidases; Vertebrata; Mammalia; Sodium; Mouse; Low; Animal; Hydrolases; Digestive diseases; Intestinal disease; Colitis
• ISSN: 0022-3166; 1541-6100
• DOI: 10.1093/jn/137.11.2475

Dietary calcium (Ca) positively modulates the susceptibility to colon cancer, but its effects on related or earlier colonic pathologies, such as inflammation and mucosal dysregulation, are poorly understood. We tested the effects of differing dietary Ca levels on acute dextran sulfate sodium (DSS)-induced colitis in mice. BALB/c mice received a normal Ca (NCa) diet (0.5% Ca), a high Ca (HCa) diet (1.5% Ca), a low Ca (LCa) diet (0.05% Ca), or a very low Ca (VLCa) diet (0.009% Ca) for 3 wk. Mucosal caspases 1, 3, and 9 were assessed by Western blotting, and the histological crypt score was assessed by microscopy. Half of the mice in each group received DSS (1.5%) for 20 d in their drinking water, and disease activity was assessed. Increasing or lowering dietary Ca increased mucosal caspases (P < 0.0001 vs. NCa). Crypt scores increased with decreasing dietary Ca levels (P < 0.0001, r = -0.675), indicating that elevated caspases in LCa groups reflected early subclinical inflammation. DSS-induced disease activity was higher in mice fed low dietary Ca levels [P < 0.0001, VLCa and DSS vs. NCa and DSS (NCaDSS) and P < 0.005, LCa and DSS vs. NCaDSS], and mice from the VLCa group were moribund within 11 d of DSS administration. Those in the HCa group did not differ greatly from controls. Together, these data indicate that Ca protects against DSS-induced colitis in mice. The mechanisms are unclear, but the calcium-sensing receptor and/or luminal precipitates of calcium phosphate microparticles may be involved. Whether these observations can be extended to patients with colitis or infectious diarrhea deserves consideration.