alternative form of poly(ADP-ribose) polymerase in Drosophila melanogaster and its ectopic expression in Rat-1 cells

We here report an alternatively spliced form of PARP lacking exon 5 of the Drosophila PARP gene encoding the auto-modification domain. The alternative form of PARP (PARP II) consists 804 amino acids with a molecular weight of 92.3 kDa. The deduced amino acid sequence of PARP II was completely matche...

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Published inBiochemical and biophysical research communications Vol. 251; no. 1; pp. 35 - 40
Main Authors Kawamura, T, Hanai, S, Yokota, T, Hayashi, T, Poltronieri, P, Miwa, M, Uchida, K
Format Journal Article
LanguageEnglish
Published United States 09.10.1998
Subjects
Amino Acid Sequence
animal breeding
animal genetics
animal physiology
Animals
Apoptosis
Apoptosis - genetics
arthropods
biosynthesis
Cloning, Molecular
DNA, Complementary
DNA, Complementary - isolation & purification
Drosophila melanogaster
Drosophila melanogaster - enzymology
Drosophila melanogaster - genetics
entomology
enzymology
Fibroblasts
genetics
Isoenzymes
Isoenzymes - biosynthesis
Isoenzymes - genetics
isolation & purification
Molecular Sequence Data
Poly(ADP-ribose) Polymerases
Poly(ADP-ribose) Polymerases - biosynthesis
Poly(ADP-ribose) Polymerases - genetics
Rats
Transformation, Genetic
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Summary:We here report an alternatively spliced form of PARP lacking exon 5 of the Drosophila PARP gene encoding the auto-modification domain. The alternative form of PARP (PARP II) consists 804 amino acids with a molecular weight of 92.3 kDa. The deduced amino acid sequence of PARP II was completely matched to that of PARP I encoded by a full-length Drosophila PARP cDNA, except it lacks the region corresponding to the auto-modification domain. To examine the function of PARP II, stable transformants of Rat-1 cells in which PARP II was ectopically expressed by MMTV-LTR were isolated and characterized. After induction with dexamethasone, PARP II transformants showed slower growth and showed morphological changes with loss of spindled shape compared to cells transformed with the vector or PARP I. The PARP II-transformed cells incorporated propidium iodide after induction; however, Annexin V and TUNEL analysis indicated these changes were not due to apoptosis.
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ISSN:0006-291X
DOI:10.1006/bbrc.1998.9403