The Genome-Wide Expression Profile of 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose-Treated MDA-MB-231 Breast Cancer Cells: Molecular Target on Cancer Metabolism

• Published in: Molecules and cells Vol. 32; no. 2; pp. 123 - 132
• Main Authors: Yu, W.S., Kyung Hee University, Seoul, Republic of Korea, Jeong, S.J., Kyung Hee University, Seoul, Republic of Korea, Kim, J.H., Kyung Hee University, Seoul, Republic of Korea, Lee, H.J., Kyung Hee University, Seoul, Republic of Korea, Song, H.S., Kyung Hee University, Seoul, Republic of Korea, Kim, M.S., Tufts University, Boston, USA, Ko, E.J., Kyung Hee University, Seoul, Republic of Korea, Khil, J.H., Kyung Hee University, Seoul, Republic of Korea, Jang, H.J., Kyung Hee University, Seoul, Republic of Korea, Kim, Y.C., Kyung Hee University, Seoul, Republic of Korea, Bae, H.S., Kyung Hee University, Seoul, Republic of Korea, Chen, C.Y., Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, USA, Kim, S.H., Kyung Hee University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Springer Korean Society for Molecular and Cellular Biology 01.08.2011; 한국분자세포생물학회
• Subjects: Angiogenesis Inhibitors - pharmacology; Antineoplastic Agents - pharmacology; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Breast Neoplasms - drug therapy; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; cancer metabolism; Cell Biology; Cell Line, Tumor; CELLS; CELLULE; CELULAS; Drugs, Chinese Herbal - pharmacology; Female; Genome-Wide Association Study; Glycolysis - genetics; Humans; Hydrolyzable Tannins - pharmacology; Life Sciences; MDA-MB-231; microarray; Microarray Analysis; Molecular Targeted Therapy; PGG; Pyruvic Acid - metabolism; real-time PCR; Rhus; Tyrosine - metabolism; 생물학; cancer metabolism; PGG; microarray; real-time PCR; MDA-MB-231
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-011-2254-1

1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphenolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the significant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes.