Hormonal regulation of actin and tubulin in an epithelial cell line from Chironomus tentans

• Published in: Archives of insect biochemistry and physiology Vol. 41; no. 2; pp. 71 - 78
• Main Authors: Fretz, A, Spindler, K.D
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 1999
• Subjects: 20-hydroxyecdysone; actin; Actins - genetics; Actins - metabolism; Animals; Base Sequence; Cell Line; cell lines; Chironomidae; Chironomidae - cytology; Chironomidae - genetics; Chironomidae - metabolism; Chironomus tentans; cytoskeletal proteins; DNA Primers - genetics; ecdysterone; Ecdysterone - pharmacology; Epithelial Cells - drug effects; Epithelial Cells - metabolism; epithelium; hormonal regulation; messenger RNA; Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; tubulin; Tubulin - genetics; Tubulin - metabolism
• ISSN: 0739-4462; 1520-6327
• DOI: 10.1002/(SICI)1520-6327(1999)41:2<71::AID-ARCH3>3.0.CO;2-I

The morphogenetic changes in an epithelial cell line from Chironomus tentans that are evoked by molting hormones and molting hormone agonists are accompanied by transient changes in the concentration of actin and beta-tubulin protein and mRNA. As compared to controls, actin protein and mRNA concentrations increase by about 50%, whereas tubulin reaches maxima of 100% increase. The proportion between globular and filamentous actin remains constant after hormone treatment.