Variants of Human Papillomavirus Types 53, 58 and 66 Identified in Central Brazil

• Published in: Virus genes Vol. 26; no. 1; pp. 83 - 87
• Main Authors: Cerqueira, Daniela Marreco, Camara, Geni Noceti de Lima, da Cruz, Márcio Rojas, Silva, Evandro Oliveira, Brígido, Marcelo de Macelo, Carvalho, Luciano Gonçalves de Souza, Martins, Cláudia Renata Fernandes
• Format: Journal Article
• Language: English
• Published: United States Kluwer Academic Publishers 2003; Springer Nature B.V
• Subjects: Amino Acid Substitution; Brazil - epidemiology; Cervical Intraepithelial Neoplasia - epidemiology; Cervical Intraepithelial Neoplasia - virology; Female; Founder Effect; Genetic Variation; Humans; Mali - ethnology; Molecular Sequence Data; Papillomaviridae - classification; Papillomaviridae - genetics; Papillomaviridae - isolation & purification; Papillomavirus Infections - epidemiology; Papillomavirus Infections - virology; Phylogeny; Polymorphism, Restriction Fragment Length; Sequence Homology, Nucleic Acid; Tumor Virus Infections - epidemiology; Tumor Virus Infections - virology; Uterine Cervical Neoplasms - epidemiology; Uterine Cervical Neoplasms - virology; Brazil; Africa; Mali
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1023/A:1022386323921

The present study on molecular characterization of human papillomaviruses occurring in Central Brazil, describes two variants each of HPV-53 and HPV-58 and one variant of HPV-66 detected in samples from smears of women showing cervical intraepithelial neoplasia grade II (CIN II). Samples were assayed by PCR using MY09/MY11 consensus primers, followed by restriction fragment length polymorphism typing. The five isolates showed atypical restriction fragment length profile and MY09/MY11 L1 PCR products were subsequently sequenced. Isolate Bsb-02 and Bsb-08 showed, respectively, 99% similarity to HPV-58 IS404 and 100% to HPV-58 IS417 previously described in the African Continent. Isolates Bsb-61 and Bsb-63 showed 98% similarity to HPV-53, and isolate Bsb-68, 97% similarity to HPV-66. Amino acid substitutions were found in two samples: one in Bsb-02 (T to N) at position 375 and the other in Bsb-61 (S to C) at position 343. Although all the substitutions in Bsb-68 proved to be silent, this sample showed the highest value of pairwise evolutionary distance (2.05%). In countries such as Brazil, where the virus prevalence is high and ethnicity, as well as socio-demographic characteristics, vary according to different regions, HPV variability must be wider and not yet clearly defined.