A highly conserved alpha-tubulin sequence from Prunus amygdalus

The sequence of an alpha-tubulin from Prunus amygdalus has been obtained by cDNA cloning. When this sequence is compared to that of the Tub alpha 1 gene from maize it shows a very high degree of similarity, much higher than any of the alpha-tubulin sequences reported so far from plants. The expressi...

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Bibliographic Details
Published inPlant molecular biology Vol. 22; no. 5; p. 913
Main Authors Stöcker, M, Garcia-Mas, J, Arús, P, Messeguer, R, Puigdomènech, P
Format Journal Article
LanguageEnglish
Published Netherlands 01.08.1993
Subjects
adn
Amino Acid Sequence
Blotting, Northern
Blotting, Southern
clonacion
clonage
cloning
Cloning, Molecular
Conserved Sequence
dna
expresion genica
expression des genes
gene
gene expression
genes
Molecular Sequence Data
nucleotide sequence
Plants - genetics
prunus amygdalus
secuencia nucleica
Sequence Homology, Amino Acid
sequence nucleique
Tubulin - genetics
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Summary:The sequence of an alpha-tubulin from Prunus amygdalus has been obtained by cDNA cloning. When this sequence is compared to that of the Tub alpha 1 gene from maize it shows a very high degree of similarity, much higher than any of the alpha-tubulin sequences reported so far from plants. The expression of this gene is high in the stages of seed development where a high divisional activity is present. It is preferentially expressed in the radicular tissues as it is gene Tub alpha 1 in maize. Southern analysis indicates that this gene may form a subfamily of alpha-tubulin genes having similar sequence and tissue specificity and existing at least in maize and in Prunus.
Bibliography:F30
F
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00027377