The Drosophila melanogaster 60A chromosomal division is extremely dense with functional genes: their sequences, genomic organization, and expression

• Published in: Genomics (San Diego, Calif.) Vol. 57; no. 1; pp. 43 - 56
• Main Authors: Lukacsovich, T. (Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.), Asztalos, Z, Juni, N, Awano, W, Yamamoto, D
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 01.04.1999
• Subjects: Acetyl-CoA C-Acetyltransferase - genetics; Amino Acid Sequence; AMINO ACID SEQUENCES; Animals; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Biological and medical sciences; Blotting, Northern; CHEMICAL COMPOSITION; CHROMOSOME; CHROMOSOMES; Classical genetics, quantitative genetics, hybrids; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; CROMOSOMAS; Databases, Factual; DNA-Binding Proteins - genetics; DROSOPHILA MELANOGASTER; Drosophila melanogaster - genetics; Drosophila Proteins - genetics; EXPRESION GENICA; Expressed Sequence Tags; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENBANK/AB010260; GENBANK/AB010261; GENBANK/AB010262; GENBANK/AB010263; GENBANK/AB010264; GENBANK/AB010265; GENBANK/AB019705; GENBANK/AB019706; GENBANK/AB019707; GENE; GENE EXPRESSION; GENES; Genes, Insect; Genetics of eukaryotes. Biological and molecular evolution; Homeodomain Proteins - genetics; IDENTIFICACION; IDENTIFICATION; In Situ Hybridization; INSERTIONAL MUTAGENESIS; INSERTIONS; INTRONS; Invertebrata; MESSENGER RNA; Models, Genetic; MOLECULAR SEQUENCE DATA; Multigene Family; MUTACION; MUTANT; MUTANTES; MUTANTS; MUTATION; NUCLEOTIDE SEQUENCE; REGULATORY GENES; Restriction Mapping; SECUENCIA NUCLEOTIDICA; Sequence Analysis, DNA; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Transforming Growth Factor beta - genetics; TRANSPOSABLE ELEMENTS; Genetic mapping; Gene; Nucleotide sequence; Arthropoda; Insecta; Homology; Invertebrata; Gene expression; Transcription factor; Drosophila melanogaster; Diptera; Drosophilidae
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1999.5746

We cloned and sequenced genomic DNA contigs spanning over 45 kb, surrounding the insertion site of the P-element that is responsible for the developmental defects in the ken and barbie (ken) mutant of Drosophila. This region harbors 10 functional transcription units, in addition to the already well-characterized TGFbeta-60A gene. These include the genes, undefined 1 (UD1), UD2, and UD3, each coding for proteins of unknown function, the ken gene encoding a new Krüppel-like putative transcription factor, the fly homologues of the mammalian mitochondrial trifunctional enzyme (thiolase), and the TAR DNA-binding protein-43 (TBPH), the first nonvertebrate member of the transmembrane 4 superfamily (TM4SF) gene, a new homeodomain gene, and a gene coding for a putative nuclear binding protein (PNBP) that is homologous to maleless, and a Copia-like element. UD3 exists in an intron of the maleless homologue, yet is expressed independent of it. The UD1 and TM4SF genes orient in a tail-to-tail manner with their 3' untranslated region sequences overlapping over 44 nucleotides. Thus the partial overlap and intraintronic organization permitted dense packing of the functional genes within a short segment of the genome.