Whitefly (Hemiptera: Aleyrodidae) binding site for imidacloprid and related insecticides: a putative nicotinic acetylcholine receptor

Imidacloprid is used extensively to control sweetpotato whiteflies, Bemisia argentifolii Bellows and Perring [also known as B. tabaci (Gennadius) biotype B]. As a radioligand, [3H]imidacloprid binds rapidly to a single class of high-affinity sites in membrane preparations from whole adult whiteflies...

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Bibliographic Details
Published inJournal of economic entomology Vol. 90; no. 4; p. 879
Main Authors Chao, S.L, Dennehy, T.J, Casida, J.E
Format Journal Article
LanguageEnglish
Published England 01.08.1997
Subjects
ACETILCOLINA
ACETYLCHOLINE
ANALOGS
Animals
BEMISIA ARGENTIFOLII
Binding Sites
CHOLINERGIC RECEPTORS
FARMACOLOGIA
Hemiptera
Imidazoles - chemistry
Imidazoles - metabolism
INHIBITORS
Insecta - metabolism
INSECTICIDAS
INSECTICIDE
INSECTICIDES
Insecticides - chemistry
Insecticides - metabolism
Kinetics
MEMBRANES
Molecular Structure
Neonicotinoids
NEUROPHARMACOLOGY
NICOTINA
NICOTINE
Nitro Compounds
PHARMACOLOGIE
PHARMACOLOGY
Receptors, Nicotinic - metabolism
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Summary:Imidacloprid is used extensively to control sweetpotato whiteflies, Bemisia argentifolii Bellows and Perring [also known as B. tabaci (Gennadius) biotype B]. As a radioligand, [3H]imidacloprid binds rapidly to a single class of high-affinity sites in membrane preparations from whole adult whiteflies with an apparent dissociation constant of 2 nM and maximal binding capacity of 101 fmol/mg protein. Three related compounds (the nitromethylene analog of imidacloprid, acetamiprid, and nitenpyram) inhibit [3H]imidacloprid binding by 50% at 0.40, 2.9, and 57 nM, respectively. The pharmacological profile of the binding site (examined with imidacloprid and the analogs listed above, and nicotine, alpha-bungarotoxin, carbachol, acetylcholine [with paraoxon], and atropine) is consistent with that anticipated for a nicotinic acetylcholine receptor and correlates well with binding results for house fly, Musca domestica L., head membranes under the same conditions. Thus, [3H]imidacloprid is a suitable radioligand to investigate the putative nicotinic acetylcholine receptor of Bemisia and the possible modifications of this target site associated with selection of resistant strains
Bibliography:H10
1997066614
ISSN:0022-0493
1938-291X