Demonstration of infectious salmon anaemia (ISA) viral antigens in cell cultures and tissue sections [ISAV, indirect fluorescent antibody technique]

• Published in: Veterinary research (Paris) Vol. 26; no. 5-6; pp. 499 - 504
• Main Authors: Falk, K. (Central Veterinary Laboratory, Oslo (Norvege). Department of Virololgy), Dannevig, B.H
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Les Ulis EDP Sciences 1995
• Subjects: Anemia - diagnosis; Anemia - pathology; Anemia - veterinary; ANIMAL VIRUSES; Animals; Antibodies; Antibodies, Monoclonal; Antigens, Viral - analysis; Biological and medical sciences; CELL CULTURE; Cell Line; CULTIVO DE CELULAS; CULTURE DE CELLULE; Endothelium, Vascular - pathology; Endothelium, Vascular - virology; Fish Diseases; Fluorescent Antibody Technique, Indirect; Fundamental and applied biological sciences. Psychology; Heart - virology; IDENTIFICACION; IDENTIFICATION; IMMUNOFLUORESCENCE; infectious salmon anemia virus; INMUNOFLUORESCENCIA; Kidney; Lentivirus Infections - diagnosis; Lentivirus Infections - pathology; Lentivirus Infections - veterinary; Mice; Mice, Inbred BALB C - immunology; Microbiology; Myocardium - pathology; Rabbits - immunology; SALMO SALAR; Salmon - virology; Techniques used in virology; Virology; VIRUS DE LOS ANIMALES; VIRUS DES ANIMAUX; Antigen; Tissue extract; Virus; Cell culture; Antiserum; Salmon; Monoclonal antibody; Indirect immunofluorescence; Detection; Immunological method
• ISSN: 0928-4249; 1297-9716

Rabbit polyclonal antibodies and mouse monoclonal antibodies (MAb) directed against infectious salmon anaemia virus (ISAV) were produced using a virus prepared in a newly established cell line culture developed from Atlantic salmon head kidney (SHK-1) cells. These antibodies were used to establish an indirect fluorescent antibody test for the detection of viral antigens in cell cultures and tissue cryosections. Specific fluorescence was detected in ISAV-infected cell cultures using rabbit polyclonal and MAb. One selected MAb produced specific staining for ISAV in the tissue sections from all the examined organs of ISAV-infected Atlantic salmon 20 d post infection. Fluorescence was mainly found in the endothelial cells and in single cells scattered throughout the parenchyma of the organs.