Effects of phosphorus supply on in vitro growth and phosphatase activity of Frankia isolates from Casuarina

• Published in: Plant and soil Vol. 189; no. 1; pp. 75 - 79
• Main Authors: Yang, Y. (James Cook Univ., Townsville, Qld. (Australia). Dept. of Microbiology and Immunology), Shipton, W.A, Reddell, P
• Format: Journal Article
• Language: English
• Published: Dordrecht Kluwer Academic Publishers 01.02.1997; Springer; Springer Nature B.V
• Subjects: ABONOS FOSFATADOS; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Agrology; Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; Bacteriology; Biochemistry and biology; Biological and medical sciences; Biotechnology; CASUARINA; Chemical, physicochemical, biochemical and biological properties; CRECIMIENTO; CROISSANCE; ENGRAIS PHOSPHATE; Enzymes; ENZYMIC ACTIVITY; FOSFORO; FRANKIA; Fundamental and applied biological sciences. Psychology; GROWTH; Growth, nutrition, cell differenciation; Microbial ecology; Microbiology; Phosphatases; PHOSPHATE FERTILIZERS; Phosphates; PHOSPHORE; PHOSPHORUS; Physics, chemistry, biochemistry and biology of agricultural and forest soils; Plant growth; Plants; Rhizobium; Soil; Soil fertility; Soil science; Sporangia; Symbiosis; VIABILIDAD; VIABILITE; VIABILITY; Australia; Oceania; Queensland; Alkaline phosphatase; Microbiology; Soil fertility; Growth; Tropical zone; Phosphoric monoester hydrolases; Supply; Frankiaceae; Casuarina equisetifolia; Esterases; Inorganic element; Frankia; Actinomycetales; Enzymatic activity; Dicotyledones; Angiospermae; Bacteria; Hardwood forest tree; Diazotrophy; Laboratory study; Symbionte; Microorganism growth; Nutrition; Enzyme; Phosphorus; In vitro; Strain; Casuarinaceae; Forestry; Soil biology; Hydrolases; Isolate; Actinomycetes; Spermatophyta; Soil plant relation; Acid phosphatase; Viability
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1023/A:1004238021805

An in vitro experiment was conducted to investigate the effects of different sources and levels of P supply on growth, viability and phosphatase activity of three tropical Frankia strains isolated from Casuarina. P concentration for optimum growth was between 0.1 and 10.0 µM in the absence of external combined nitrogen. Specific viability was not influenced by P supply. Morphological features of Frankia, such as hyphal length and vesicle numbers, were found to largely mirror growth. Phosphatase activity was detected in all three Frankia strains and was highest when P was omitted from the culture solution. There were more than 10-fold differences between the Frankia strains in the level of phosphatase activities shown. This study suggested that soils low in P are unlikely to restrict micro-symbiont growth activity.