Lipid isolation and fatty acid analysis in Saccharomyces cerevisiae. Comparison of different methods

Several methods for lipid isolation and fatty acid analysis in two different strains of Saccharomyces cerevisiae were compared. As the most effective procedure the extraction with tetrachlormethane-diethyl ether-petrol ether mixture with an acid digestion step followed by fatty acid analysis by capi...

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Bibliographic Details
Published inBiotechnology techniques Vol. 12; no. 8; pp. 621 - 625
Main Authors Lamacka, M, Sajbidor, J, Bohov, P
Format Journal Article
LanguageEnglish
Published London Chapman and Hall 1998
Subjects
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Others
plant biochemistry
plant physiology
Saccharomyces cerevisiae
Various methods and equipments
Yeast
Extraction process
Lipids
Solvent extraction
Fatty acids
Fungi
Analysis method
Ascomycetes
Qualitative analysis
Saccharomyces cerevisiae
Comparative study
Thallophyta
Quantitative analysis
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Summary:Several methods for lipid isolation and fatty acid analysis in two different strains of Saccharomyces cerevisiae were compared. As the most effective procedure the extraction with tetrachlormethane-diethyl ether-petrol ether mixture with an acid digestion step followed by fatty acid analysis by capillary GC was found. Other methods underestimated both the lipid content and the content of fatty acids.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1008832013587