Oligosaccharides synthesis by free and immobilized beta-galactosidases from Thermus aquaticus YT-1

• Published in: Biotechnology letters Vol. 17; no. 10; pp. 1077 - 1080
• Main Authors: Berger, J.L, Lee, B.H, Lacroix, C
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.01.1995
• Subjects: beta-galactosidase; Biological and medical sciences; biosynthesis; Biotechnology; Fundamental and applied biological sciences. Psychology; galactooligosaccharides; immobilization; Immobilization of enzymes and other molecules; Immobilization techniques; lactose; Methods. Procedures. Technologies; oligosaccharides; thermophilic bacteria; Thermus aquaticus; Performance evaluation; Thermus aquaticus; Enzyme; Free form; Oligosaccharide; Biosynthesis; Enzymatic activity; Glycosidases; Production; Bacteria; Hydrolases; O-Glycosidases; Immobilized enzyme; β-Galactosidase
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/BF00143104

Galactooligosaccharides (GalOS) production from a lactose solution (16% w/v) was studied at 70 degrees C, pH 4.6 and 6.0 with free or immobilized beta-galactosidases of Thermus aquaticus YT-1. Synthesis of OS and lactose conversion was significantly improved when using the immobilized preparation. At pH 4.6, OS accounted for approx. 35% of the total carbohydrates with a conversion rate of lactose of 80%. The general formula of OS synthesized in this way was Gal-(Gal)n-Glu with n being equal to 1 or 2 and a beta anomeric configuration of the D-galactosyl moiety.