Cellulase-free xylanase production from an alkalophilic Bacillus species

• Published in: World journal of microbiology & biotechnology Vol. 8; no. 6; pp. 627 - 631
• Main Authors: Balakrishnan, H, Dutta-Choudhury, M, Srinivasan, M.C, Rele, M.V
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.01.1992
• Subjects: Bacillus; Bacillus (bacteria); Biological and medical sciences; biosynthesis; Biotechnology; bleaching; enzyme activity; Enzyme engineering; Fermentation; Fundamental and applied biological sciences. Psychology; hydrolysis; Methods. Procedures. Technologies; O-glycoside hydrolases; Production of selected enzymes; wood pulp; xylan; Culture medium; Enzyme; Alkalophily; Endo-1,4-β-xylanase; Bacillaceae; Characterization; Bacillales; Enzymatic activity; Cellulase; Substrate specificity; Bacillus; Microorganism culture; Bacteria; Kinetics
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/BF01238802

An alkalophilic Bacillus (NCL-87-6-10, NCIM 2128), with a high productivity for extracellular xylanase (EC 3.2.1.8) and free of cellulase, was isolated from soil containing coconut fibre detritus. When grown on a wheat bran/yeast extract medium in submerged culture for 48 h, it produced 100 to 120 IU of enzyme activity per ml. The crude enzyme consists of two fractions of apparent mol sizes of approx 10.4 and 29 kDa in the proportion of 90:10, as determined by native gel exclusion chromatography. Optimum activity of the xylanase was at 60 degree C and pH 8.0. A two fold increase in enzyme activity was obtained when reducing agents, thioethanol and dithiothreitol, were included in the assay.