Immobilization of Glucose Oxidase on Multi-Wall Carbon Nanotubes for Biofuel Cell Applications

• Published in: Journal of microbiology and biotechnology Vol. 15; no. 2; pp. 234 - 238
• Main Authors: Jung, S.K. (University of Ulsan, Ulsan, Republic of Korea), Chae, Y.R. (University of Ulsan, Ulsan, Republic of Korea), Yoon, J.M. (University of Ulsan, Ulsan, Republic of Korea), Cho, B.W. (Korea Institute of Science and Technology, Seoul, Republic of Korea), Ryu, K.G. (University of Ulsan, Ulsan, Republic of Korea), E-mail: kgryu@mail.ulsan.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.04.2005; 한국미생물·생명공학회
• Subjects: Biological and medical sciences; Biotechnology; Carbon nanotubes; Fundamental and applied biological sciences. Psychology; GLUCOSA OXIDASA; GLUCOSE OXIDASE; GLUCOSE OXYDASE; IMMOBILISATION; IMMOBILIZATION; INMOVILIZACION; 생물학; Glucose oxidase; Enzyme; Nanotube; Immobilization; Carbon nanotubes; Biofuel; Oxidoreductases; Application; Carbon
• ISSN: 1017-7825

Glucose oxidase was immobilized on the carboxylated multi-wall carbon nanotubes (MWNT-COOHs) in the presence of a coulping reagent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. Significant amounts of glucose oxidase were also immobilized on MWNT-COOHs without the coupling reagent. Various conditions for the immobilization of glucose oxidase were optimized. Optimal pH for the maximal activity of the immobilized glucose oxidase shifted to 7 from the optimal pH of 6 for the maximal activity of free enzyme due to the carboxyl groups on the surface of MWNT-COOHs.