Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium

• Published in: The journal of microbiology Vol. 43; no. 2; pp. 204 - 208
• Main Authors: Yoo, S.Y. (Chosun University, Gwangju, Republic of Korea), Kim, P.S. (Chosun University, Gwangju, Republic of Korea), Hwang, H.K. (Chosun University, Gwangju, Republic of Korea), Lim, S.H. (Chosun University, Gwangju, Republic of Korea), Kim, K.W. (Chosun University, Gwangju, Republic of Korea), Choe, S.J. (Seoul National University, Seoul, Republic of Korea), Min, B.M. (Seoul National University, Seoul, Republic of Korea), Kook, J.K. (Chosun University, Gwangju, Republic of Korea), E-mail: jkkook@chosun.ac.kr
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물학회 01.04.2005
• Subjects: 16S rDNA; Adolescent; Adult; Agar; Bacitracin; Culture Media; dental plaque; Dental Plaque - microbiology; Humans; mitis-salivarius bacitracin agar; non-mutans streptococci organism; STREPTOCOCCUS; Streptococcus - isolation & purification; 생물학
• ISSN: 1225-8873; 1976-3794

The objective of this study was to both isolate and identify non-mutans streptococci organisms (non-­MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects, 12 colonies, derived from 10 subjects, were identified as non-MSO.