Characterization, Cloning and Expression of the Ferritin Gene from the Korean Polychaete, Periserrula leucophryna

• Published in: The journal of microbiology Vol. 44; no. 1; pp. 54 - 63
• Main Authors: Jeong, B.R. (Daegu University, Gyeongsan, Republic of Korea), Chung, S.M. (The Catholic University of Korea, Seoul, Republic of Korea), Baek, N.J. (Daegu University, Gyeongsan, Republic of Korea), Koo, K.B. (Daegu University, Gyeongsan, Republic of Korea), Baik, H.S. (Pusan National University, Busan, Republic of Korea), Joo, H.S. (Inha University, Incheon, Republic of Korea), Chang, C.S. (Inha University, Incheon, Republic of Korea), Choi, J.W. (Daegu University, Gyeongsan, Republic of Korea), E-mail: chjawo@daegu.ac.kr
• Format: Journal Article
• Language: English
• Published: Korea (South) 01.02.2006
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Escherichia coli; Escherichia coli - enzymology; Escherichia coli - genetics; FERRITIN; FERRITINE; Ferritins - chemistry; Ferritins - genetics; Ferritins - metabolism; Gene Library; Humans; iron responsive element; Iron-Regulatory Proteins - chemistry; Iron-Regulatory Proteins - metabolism; Korea; Mice; Molecular Sequence Data; Periserrula leucophryna; Polychaeta - genetics; Polychaeta - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA; Korea
• ISSN: 1225-8873; 1976-3794

Ferritin is a major eukaryotic protein and in humans is the protein of iron storage. A partial gene fragment of ferritin (255 bp) taken from the total RNA of Periserrula leucophryna, was amplified by RT-PCR using oligonucleotide primers designed from the conserved metal binding domain of eukaryotic ferritin and confirmed by DNA sequencing. Using the ∨32P-labeled partial ferritin cDNA fragment, 28 different clones were obtained by the screening of the P. leucophryna cDNA library prepared in the Uni-ZAP XR vector, sequenced and characterized.