Isolation and partial amino acid sequence of the trypsin inhibitor from the seeds of Cucurbita maxima

Trypsin inhibitor from squash (Cucurbita maxima) seed was extracted with 0.1 M-acetate buffer, pH 4.5, purified on immobilized trypsin, and separated by SE-Sephadex C-50 chromatography into three active fractions. All of them inhibited trypsin to the same extent, showed no antichymotrypsin or antika...

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Bibliographic Details
Published inActa biochimica polonica Vol. 27; no. 3/4; pp. 371 - 382
Main Authors Polanowski, A, Wilusz, T, Nienartowicz, B, Cieslar, E, Slominska, A, Nowak, K
Format Journal Article
LanguageEnglish
Published Poland 1980
Subjects
Amino Acid Sequence
Amino Acids - analysis
Cucurbita maxima
isolation
Molecular Weight
nonfood plant products
Pepsin A
Peptide Fragments - analysis
plant biochemistry
plant physiology
Seeds - analysis
Trypsin
trypsin inhibitors
Trypsin Inhibitors - isolation & purification
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Summary:Trypsin inhibitor from squash (Cucurbita maxima) seed was extracted with 0.1 M-acetate buffer, pH 4.5, purified on immobilized trypsin, and separated by SE-Sephadex C-50 chromatography into three active fractions. All of them inhibited trypsin to the same extent, showed no antichymotrypsin or antikallikrein activity, had a similar molecular weight (about 3300), and contained no tryptophan, phenylalanine or threonine. The partial amino acid sequence of tryptic and peptic peptides of fraction III was determined by Edman degradation procedure.
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ISSN:0001-527X