Glycosylation of Flavonoids with E. coli Expressing Glycosyltransferase from Xanthomonas campestris

• Published in: Journal of microbiology and biotechnology Vol. 17; no. 3; pp. 539 - 542
• Main Authors: Kim, J.H. (Konkuk University, Seoul, Republic of Korea), Kim, B.G. (Konkuk University, Seoul, Republic of Korea), Kim, J.A. (Konkuk University, Seoul, Republic of Korea), Park, Y.H. (Konkuk University, Seoul, Republic of Korea), Lee, Y.J. (Konkuk University, Seoul, Republic of Korea), Lim, Y.H. (Konkuk University, Seoul, Republic of Korea), Ahn, J.H. (Konkuk University, Seoul, Republic of Korea), E-mail: jhahn@konkuk.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.03.2007
• Subjects: Biological and medical sciences; Biotechnology; Biotechnology - methods; biotransformation; Chromatography, High Pressure Liquid; Escherichia coli - enzymology; Escherichia coli - genetics; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; Flavonoids - chemistry; Flavonoids - metabolism; Fundamental and applied biological sciences. Psychology; GLICOSILTRANSFERASAS; Glycosylation; GLYCOSYLTRANSFERASE; GLYCOSYLTRANSFERASES; Glycosyltransferases - genetics; Glycosyltransferases - metabolism; Luteolin - chemistry; Luteolin - metabolism; Magnetic Resonance Spectroscopy; Quercetin - chemistry; Quercetin - metabolism; Xanthomonas campestris - enzymology; Xanthomonas campestris - genetics; Pseudomonadales; Xanthomonas campestris; Enzyme; Transferases; Biotransformation; glycosyltransferase; Glycosyltransferases; Bacteria; Pseudomonadaceae; Glycosylation; flavonoids; Flavonoid
• ISSN: 1017-7825

Glycosyltransferase family 1 (UGT) uses small chemicals including phenolics, antibiotics, and alkaloids as substrates to have an influence in biological activities. A glycosyltransferase (XcGT-2) from Xanthomonas campestris was cloned and consisted of a 1,257 bp open reading frame encoding a 45.5 kDa protein. In order to use this for the modification of phenolic compounds, XcGT-2 was expressed in Escherichia coli as a glutathione S-transferase fusion protein. With the E. coli transformant expressing XcGT-2, biotransformation of flavonoids was carried out.