Functional Characteristics of Cyclodextrin Glucanotransferase from Alkalophilic Bacillus sp. BL-31 Highly Specific for Intermolecular Transglycosylation of Bioflavonoids

• Published in: Journal of microbiology and biotechnology Vol. 17; no. 9; pp. 1550 - 1553
• Main Authors: Go, Y.H. (Kyungpook National University, Daegu, Republic of Korea), Kim, T.K. (Kyungpook National University, Daegu, Republic of Korea), Lee, K.W. (Kyungpook National University, Daegu, Republic of Korea), Lee, Y.H. (Kyungpook National University, Daegu, Republic of Korea), E-mail: leeyh@knu.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.09.2007
• Subjects: alkalophilic; Bacillus - enzymology; Bacillus sp. BL-31; Biological and medical sciences; Biotechnology; Chromatography, High Pressure Liquid; cyclodextrin glucanotransferase; Flavanones - metabolism; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; Flavonoids - biosynthesis; Flavonoids - metabolism; Fundamental and applied biological sciences. Psychology; Glucosyltransferases - isolation & purification; Glucosyltransferases - metabolism; glycosyl naringin; Glycosylation; Hesperidin - metabolism; intermolecular transglycosylation; Molecular Structure; Rutin - metabolism; Cyclodextrin glucanotransferase; Enzyme; Alkalophily; bioflavonoids; Transferases; Glycosyltransferases; Bacillaceae; Flavonoid; Transglycosylation; intermolecular transglycosylation; Bacillales; Bacillus; alkalophilic Bacillus sp. BL-31; Bacteria; Cyclomaltodextrin glucanotransferase; Hexosyltransferases; glycosyl naringin
• ISSN: 1017-7825

The functional characteristics of a β-cyclodextrin glucanotransferase (CGTase) excreted from alkalophilic Bacillus sp. BL-31 that is highly specific for the intermolecular transglycosylation of bioflavonoids were investigated. The new β-CGTase showed high specificities for glycosyl acceptor bioflavonoids, including naringin, rutin, and hesperidin, and especially naringin. The transglycosylation of naringin into glycosyl naringin was then carried out under the conditions of 80 units of CGTase per gram of maltodextrin, 5 g/l of naringin, 25 g/l of maltodextrin, and 1 mM Mn²+ ion at 40℃ for 6 h, resulting in a high conversion yield of 92.1%.