ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium

The establishment of reliable serological methods for cysticercosis in pigs is important for the surveillance, control and prevention of taeniosis/cysticercosis in humans as well as in pigs to prevent economic loss. Both ELISA and immunoblot using glycoproteins (GPs) purified by a single step of pre...

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Bibliographic Details
Published inJournal of helminthology Vol. 73; no. 4; pp. 363 - 365
Main Authors Ito, A, Plancarte, A, Nakao, M, Nakaya, K, Ikejima, T, Piao, Z.X, Kanazawa, T, Margono, S.S
Format Journal Article
LanguageEnglish
Published England 01.12.1999
Subjects
Animals
cysticercosis
Cysticercosis - diagnosis
Cysticercosis - veterinary
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Glycoproteins - immunology
Humans
Immunoblotting - methods
Immunoblotting - veterinary
infection
serodiagnosis
Swine
Swine Diseases - diagnosis
Taenia solium
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Summary:The establishment of reliable serological methods for cysticercosis in pigs is important for the surveillance, control and prevention of taeniosis/cysticercosis in humans as well as in pigs to prevent economic loss. Both ELISA and immunoblot using glycoproteins (GPs) purified by a single step of preparative iso-electric focusing, which are highly useful for human cysticercosis, have been applied for a serological study in pigs naturally infected with Taenia solium. All sera from pigs showed similar responses to those in human cysticercosis. Therefore, it is expected that both ELISA and immunoblots using GPs would be useful in differentiating infected pigs from uninfected ones.
ISSN:0022-149X
1475-2697
DOI:10.1017/S0022149X9900061X