Isolation of a retrovirus from cultured equine sarcoid tumor cells

A virus with the morphologic and biochemical properties of the family Retroviridae has been isolated from cultured cells explanted from a malignant tumor induced by intradermal inoculation of equine sarcoid cells into a combined immunodeficient Arabian foal. By electron microscopy, intracytoplasmic,...

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Bibliographic Details
Published inAmerican journal of veterinary research Vol. 43; no. 5; p. 804
Main Authors Cheevers, W.P, Roberson, S.M, Brassfield, A.L, Davis, W.C, Crawford, T.B
Format Journal Article
LanguageEnglish
Published United States 01.05.1982
Subjects
Animals
Cells, Cultured
Horse Diseases - microbiology
Horses
Magnesium - metabolism
Manganese - metabolism
Microscopy, Electron
Retroviridae - isolation & purification
Retroviridae - metabolism
Retroviridae - ultrastructure
Skin Neoplasms - microbiology
Skin Neoplasms - veterinary
Uridine - metabolism
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Summary:A virus with the morphologic and biochemical properties of the family Retroviridae has been isolated from cultured cells explanted from a malignant tumor induced by intradermal inoculation of equine sarcoid cells into a combined immunodeficient Arabian foal. By electron microscopy, intracytoplasmic, extracellular, and budding particles measuring 89 to 120 nm with electron-lucent cores were seen. Virus purified from the medium of cultured cells had a buoyant density of 1.15 g/cm3 in isopycnic sucrose-gradient centrifugation, incorporated radiolabeled uridine but not thymidine, and had constitutive RNA-dependent DNA polymerase which required Mn2+ for optimal endogenous activity.
Bibliography:8236582
L73
ISSN:0002-9645
1943-5681