A Brevibacterium linens pRBL1 replicon functional in Corynebacterium glutamicum

• Published in: Plasmid Vol. 36; no. 1; pp. 36 - 41
• Main Authors: Ankri, S. (Universite de Paris-Sud, Orsay, France.), Bouvier, I, Reyes, O, Predali, F, Leblon, G
• Format: Journal Article
• Language: English
• Published: United States 01.07.1996
• Subjects: Amino Acid Sequence; BACTERIA; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Brevibacterium - genetics; BREVIBACTERIUM LINENS; Cloning, Molecular; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Corynebacterium - genetics; Corynebacterium glutamicum; CULTIVOS INICIADORES; CULTURE STARTER; FABRICACION DEL QUESO; FABRICATION FROMAGERE; Genetic Vectors - physiology; GENETICA; GENETIQUE; Molecular Sequence Data; Mycobacterium fortuitum; Neisseria gonorrhoeae; PLASMIDE; PLASMIDIOS; Plasmids - physiology; PROTEINAS; PROTEINE; REPLICACION; REPLICATION; Replicon - physiology; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE
• ISSN: 0147-619X; 1095-9890
• DOI: 10.1006/plas.1996.0029

Brevibacterium linens RBL strain cryptic plasmid pRBL1 (8.0 kb) is described. A region involved in pRBL1 autonomous replication in Corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. This region encodes for a hypothetical 310-amino acid (aa) protein closely related to the replicases of plasmids pXZ10142 (C. glutamicum) and pAL5000 (Mycobacterium fortuitum). The 310-aa protein also shows significant homology to proteins of pColE5-099 (Shigella sonnei) and pJD1 (Neisseria gonorrhoea). At least one of these proteins, the Rep protein of pColE5-099, is known to be involved in theta replication