Bovine leukocyte adhesion deficiency: in vitro assessment of neutrophil function and leukocyte integrin expression

Bovine leukocyte adhesion deficiency (BLAD) was identified in a two-month-old Holstein heifer calf using DNA-polymerase chain reaction analysis of the affected calf and other clinical parameters. Neutrophil integrin expression (CD18, CD11a, CD11c), aggregation, and transendothelial migration were st...

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Published inCanadian journal of veterinary research Vol. 58; no. 2; pp. 127 - 133
Main Authors Olchowy, T.W.J, Bochsler, P.N, Neilsen, N.R, Welborn, M.G, Slauson, D.O
Format Journal Article
LanguageEnglish
Published Canada 01.04.1994
Subjects
aggregation
Animals
Antigens, CD - biosynthesis
Cattle
Cattle Diseases - immunology
CD11 Antigens
CD18 Antigens
Cell Adhesion
Cell Aggregation
Cell Migration Inhibition
Chemotaxis, Leukocyte
dairy cattle
Female
Flow Cytometry - veterinary
Holstein
Immune System Diseases - genetics
Immune System Diseases - immunology
Immune System Diseases - veterinary
immunosuppression (physiological)
Integrins - biosynthesis
Leukocytes - immunology
neutrophils
Neutrophils - immunology
Polymerase Chain Reaction - veterinary
Receptors, Leukocyte-Adhesion - biosynthesis
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Summary:Bovine leukocyte adhesion deficiency (BLAD) was identified in a two-month-old Holstein heifer calf using DNA-polymerase chain reaction analysis of the affected calf and other clinical parameters. Neutrophil integrin expression (CD18, CD11a, CD11c), aggregation, and transendothelial migration were studied in vitro. Neutrophils were isolated from the affected calf and from normal, healthy, age-matched control Holstein calves. Neutrophils isolated from the affected BLAD calf had decreased expression of leukocyte integrins on their cell surface, decreased ability to aggregate in response to chemotactic stimuli, and decreased ability to migrate across bovine endothelial cell monolayers in vitro. Transendothelial migration of neutrophils from normal calves was reduced to levels comparable to the BLAD neutrophils by treatment with an anti-CD18 monoclonal antibody (MAb 60.3). Peripheral-blood lymphocytes from the BLAD calf also expressed negligible levels of leukocyte integrins, similar to their neutrophil counterparts. Our experimental findings in vitro correlate well with the clinical observations of decreased leukocyte trafficking and diminished host defense in leukocyte adhesion-deficient animals. The syndrome of BLAD may be a suitable model for one of the human leukocyte adhesion deficiency disorders.
ISSN:0830-9000