Localisation of cis elements in the promoter of a wheat alpha-Amy2 gene

• Published in: Plant molecular biology Vol. 19; no. 6; p. 903
• Main Authors: Huttly, A.K. (Bristol Univ. (United Kingdom). Dept. of Agricultural Sciences), Phillips, A.L, Tregear, J.W
• Format: Journal Article
• Language: English
• Published: Netherlands 01.09.1992
• Subjects: ACIDO GIBERELICO; ALFA AMILASA; ALPHA AMYLASE; alpha-Amylases - genetics; alpha-Amylases - metabolism; Base Sequence; Chimera - genetics; Cloning, Molecular; DNA; Enhancer Elements, Genetic; EXPRESION GENICA; EXPRESSION DES GENES; GENE; GENE EXPRESSION; Gene Expression Regulation; GENES; GIBBERELLINE; Molecular Sequence Data; Mosaic Viruses - genetics; Mutagenesis; Promoter Regions, Genetic; PROTOPLASTE; PROTOPLASTOS; PROTOPLASTS; TRITICUM; Triticum - enzymology; Triticum - genetics
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/BF00040523

A functional analysis of the promoter from the wheat alpha-amylase gene alpha-Amy2/54 is described. Mutant alpha-Amy2/54 promoters containing replacements or deletions were constructed and their ability to direct expression of the reporter gene beta-glucuronidase (GUS) in gibberellin-responsive oat aleurone protoplasts analysed. Chimaeric promoters using regions of the cauliflower mosaic virus (CaMV) 35S and alpha-Amy2/54 promoters were also analysed. The results suggest that at least three regions within the alpha-Amy2/54 promoter contain cis elements that are necessary for high-level gibberellin-regulated transcription. Fusion of 1.8 kb of promoter sequence upstream from -117 bp to a minimal (-55 CaMV 35S) promoter gave rise to hormone-independent expression implying that the region 3' to -117 bp contains an element which represses transcription in the absence of gibberellin or presence of abscisic acid.