Cloning and expression analysis of a viroid-induced peroxidase from tomato plants

• Published in: Molecular plant-microbe interactions Vol. 6; no. 6; pp. 790 - 794
• Main Authors: Vera, P, Tornero, P, Conejero, V
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.11.1993
• Subjects: Agronomy. Soil science and plant productions; Amino Acid Sequence; AMINO ACID SEQUENCES; ARN MENSAJERO; ARN MESSAGER; Base Sequence; Biological and medical sciences; cevi-1 gene; CITRUS EXOCORTIS VIROID; Cloning, Molecular; COMPLEMENTARY DNA; DEFENSE MECHANISMS; DNA, Complementary; ENFERMEDADES DE LAS PLANTAS; Enzyme Induction; EXPERIMENTACION IN VIVO; EXPERIMENTATION IN VIVO; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; genbank/x71593; GENE; GENE EXPRESSION; GENES; Genes. Genome; IN VIVO EXPERIMENTATION; INFECCION; INFECTION; LYCOPERSICON ESCULENTUM; MALADIE DES PLANTES; MESSENGER RNA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; PATHOGENESE; PATHOGENESIS; PATOGENESIS; PEROXIDASAS; PEROXIDASES; Peroxidases - biosynthesis; Peroxidases - genetics; PEROXYDASE; PLANT DISEASES; PROTEINAS; PROTEINE; PROTEINS; SECUENCIA NUCLEICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEIQUE; STRUCTURAL GENES; Vegetables - enzymology; Vegetables - microbiology; VIROIDE; VIROIDES; VIROIDS; Viroids - physiology; Plant pathogen; Nucleotide sequence; Enzyme; Sequence alignment; Homology; Defensive response; Host virus relation; Infection; Virus; Peroxidases; Dicotyledones; Viroid; Viral disease; Citrus exocortis virus; Angiospermae; Lycopersicon esculentum; Spermatophyta; Peroxidase; Oxidoreductases; Solanaceae; Molecular cloning; Comparative study; Aminoacid sequence; Structural analysis
• ISSN: 0894-0282; 1943-7706

Differential hybridization was used to detect transcripts induced both by ethylene and by viroid infection in tomato plants. A cDNA clone encoding a putative peroxidase was isolated and characterized. DNA sequencing revealed high homology with a lignin-peroxidase from tobacco. Northern blot analysis showed specific induction of this peroxidase gene in viroid-infected plants. An increase in the level of mRNA accumulation is obtained by ethylene treatment, reinforcing the idea that ethylene is a mediator in the response of tomato plants to viroid infection.