Protein changes in response to progressive water deficit in maize: quantitative variation and polypeptide identification

• Published in: Plant physiology (Bethesda) Vol. 117; no. 4; pp. 1253 - 1263
• Main Authors: Riccardi, F. (Universite de Paris-Sud/INRA, Gif-sur-Yvette, France.), Gazeau, P, Vienne, D. de, Zivy, M
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.08.1998
• Subjects: Agronomy. Soil science and plant productions; AMINO ACID SEQUENCES; Amino acids; BIOCHEMICAL PATHWAYS; Biological and medical sciences; CHEMICAL COMPOSITION; Complementary DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Corn; DEFICIT DE HUMEDAD EN EL SUELO; DEFICIT HYDRIQUE DU SOL; Dehydration; DROUGHT STRESS; Economic plant physiology; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; Enzymes; ESTRES DE SEQUIA; FENOTIPOS; FEUILLE; Fundamental and applied biological sciences. Psychology; Gels; GENOTIPOS; GENOTYPE; GENOTYPES; HIBRIDOS; HOJAS; HYBRIDE; HYBRIDS; LEAVES; LIGNIFICACION; LIGNIFICATION; METABOLISM; METABOLISME; METABOLISMO; MOLECULAR SEQUENCE DATA; Nitrogen metabolism; Nitrogen metabolism and other ones (excepting carbon metabolism); Nutrition. Photosynthesis. Respiration. Metabolism; PHENOTYPE; PHENOTYPES; Plant physiology and development; PLANT PROTEINS; Plants; PROTEINAS; PROTEINE; PROTEINS; SOIL WATER DEFICIT; STRESS DU A LA SECHERESSE; VIA BIOQUIMICA DEL METABOLISMO; VOIE BIOCHIMIQUE DU METABOLISME; WATER STRESS; Whole Plant, Environmental, and Stress Physiology; ZEA MAYS; Monocotyledones; Zea mays; Induction; Adaptive response; Plant leaf; Gene expression; Water stress; Cereal crop; Protein; Progressive; Image analysis; Gramineae; Angiospermae; Spermatophyta; Chemical composition; Intraspecific comparison; Two dimensional electrophoresis; Biological accumulation
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.117.4.1253

Three-week-old plants of two unrelated lines of maize (Zea mays L.) and their hybrid were submitted to progressive water stress for 10 d. Changes induced in leaf proteins were studied by two-dimensional electrophoresis and quantitatively analyzed using image analysis. Seventy-eight proteins out of a total of 413 showed a significant quantitative variation (increase or decrease), with 38 of them exhibiting a different expression in the two genotypes. Eleven proteins that increased by a factor of 1.3 to 5 in stressed plants and 8 proteins detected only in stressed plants were selected for internal amino acid microsequencing, and by similarity search 16 were found to be closely related to previously reported proteins. In addition to proteins already known to be involved in the response to water stress (e.g. RAB17 [Responsive to ABA]), several enzymes involved in basic metabolic cellular pathways such as glycolysis and the Krebs cycle (e.g. enolase and triose phosphate isomerase) were identified, as well as several others, including caffeate O-methyltransferase, the induction of which could be related to lignification