SCAR Marker Linked with A1 Mating Type Locus in Phytophthora infestans

• Published in: Journal of microbiology and biotechnology Vol. 16; no. 5; pp. 724 - 730
• Main Authors: Zhang, Xuan Zhe (Kangnung National University, Kangnung, Republic of Korea), Seo, H.W. (National Institute of Highland Agriculture, RDA, Pyeongchang, Republic of Korea), Ahn, W.G. (National Institute of Highland Agriculture, RDA, Pyeongchang, Republic of Korea), Kim, B.S. (Kangnung National University, Kangnung, Republic of Korea), E-mail: bskim@kangnung.ac.kr
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.05.2006; 한국미생물·생명공학회
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; mating type; PHYTOPHTHORA INFESTANS; SCAR marker; 생물학; Fungi; Phytophthora infestans; SCAR marker; Mating type; Phycomycetes; Thallophyta
• ISSN: 1017-7825

A sequence characterized amplified region (SCAR) marker, which was tightly linked with the A1 mating type locus in Phytophthora infestans, was developed. During the random amplified polymorphic DNA-based phylogenic studies of 33 isolates of P. infestans collected from year 2002 to 2004, we found an A1 mating type-specific DNA fragment. This 573-bp DNA fragment was generated only in the genomic DNA of the A1 mating types, when OPC-5 primer was used. Based on the specific DNA sequence, we designed the primer sets for generating the A1 mating type-specific 569-bp DNA fragment.