Modulating neurogenesis in embryoid body using a selective TGF beta1/ALK inhibitor affects gene expression of embryonic stem cell-derived motor neurons

• Published in: Wētchasān sattawaphāet Vol. 43; no. 1; p. 49
• Main Authors: Nuttha Klincumhom(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Obstetrics, Gynaecology and Reproduction), Theerawat Tharasanit(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Obstetrics, Gynaecology and Reproduction), Chommanart Thongkittidilok(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Obstetrics, Gynaecology and Reproduction), Narong Tiptanavattana(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Obstetrics, Gynaecology and Reproduction), Dinnyes, Andras(Biotalentum Ltd., Godollo (Hungary)), Mongkol Techakumphu(Chulalongkorn University, Bangkok (Thailand). Faculty of Veterinary Science. Department of Obstetrics, Gynaecology and Reproduction) E-mail:mongkol.T@chula.ac.th
• Format: Journal Article
• Language: English
• Published: Bangkok Chulalongkorn University, Faculty of Veterinary Science 01.03.2013
• Subjects: Animal embryos; Body parts; Cells; Embryoid body; Embryonic stem cells; Embryos; Gene expression; Higher education; Inhibition; Mice; Motor neuron differentiation; Nervous system; Nervous system diseases; Neurogenesis; Neurons; Rodents; Spinal cord; Stem cells; TGF-beta 1 inhibitor; Tissue engineering; Transforming growth factor
• ISSN: 0125-6491

Mouse embryonic stem (ES) cells have been served as potential model for investigation of underlying mechanisms at cellular and molecular levels of neurological disorders. The improvement of motor neuron differentiation is prospected to gain more understanding aimed at overcoming several incurable motor neuron diseases. In this study, they examined the effects of selective inhibitor of TGF-Β type I receptor on the efficacy of neuronal differentiation of mouse embryonic stem cells toward motor neuron. Immunocytochemistry demonstrated that the differentiated cells expressed a neuronal marker, motor neuron progenitor marker (Olig2) and developing motor neuron progenitor (Isl-1), all of which are essential for generation of spinal motor neurons during neural tube formation. Furthermore, a small proportion of differentiated cells were also positive for choline acetyltransferase a marker for functional motor neurons. We concluded that modifying TGF-Β signaling affected the generation and differentiation fate of motor neuron progenitor cells.