Characterisation of Bifidobacterium animalis subsp. lactis BB-12 and other probiotic bacteria using genomics, transcriptomics an proteomics

A number of B. animalis subsp. lactis strains isolated from commercial probiotic products have been compared to BB-12 at the genetic level. The similarity of these strains is apparent from their DNA fingerprints (Figure 1). DNA-DNA hybridisation on microarrays designed from the BB-12 genome sequence...

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Bibliographic Details
Published inAustralian Journal of Dairy Technology Vol. 60; no. 2; pp. 84 - 92
Main Authors Garrigues, C, Stuer-Lauridsen, B, Johansen, E
Format Journal Article Conference Proceeding
LanguageEnglish
Published Melbourne Australian Society of Dairy Technology 01.07.2005
Dairy Industry Association of Australia
Subjects
Amino acids
Bacteria
Bifidobacterium animalis
bile salts
Biological and medical sciences
Biology
Carbohydrates
Enzymes
Experiments
Food industries
fructooligosaccharides
Fundamental and applied biological sciences. Psychology
Gene expression
genome
Genomes
Genomics
Immune system
Metabolism
Metabolites
Milk and cheese industries. Ice creams
Nutrients
Organisms
Physiology
Probiotics
Proteins
Proteomics
strain differences
transcription (genetics)
Actinomycetales
Probiotic
Bifidobacterium
Actinomycetaceae
Genomics
Dairy industry
Bacteria
Actinomycetes
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Summary:A number of B. animalis subsp. lactis strains isolated from commercial probiotic products have been compared to BB-12 at the genetic level. The similarity of these strains is apparent from their DNA fingerprints (Figure 1). DNA-DNA hybridisation on microarrays designed from the BB-12 genome sequence was used to further compare several of these strains. The reference in these hybridisation experiments was BB-12 labelled with Cy3, which serves as a positive control for the spot intensity.
ISSN:0004-9433