Effect of peroxisomicine A2 and T 544 of the genus Karwinskia on peroxisomes of Candida boidinii

• Published in: FEMS microbiology letters Vol. 158; no. 2; pp. 255 - 260
• Main Authors: Salazar-Aranda, R, Sepulveda-Saavedra, J, Waksman de Torres, N, Pineyro-Lopez, A, Moreno-Sepulveda, M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1998; Blackwell; Oxford University Press
• Subjects: Anthracenes - pharmacology; Anthracenone; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antifungal agents; Antineoplastic Agents, Phytogenic - pharmacology; Biocompatibility; Biological and medical sciences; Candida - drug effects; Candida - growth & development; Candida - metabolism; Candida boidinii; Cell death; Cell viability; Damage; Dimeric anthracenone; Disruption; Karwinskia; Medical sciences; medicinal plants; Methylotrophic yeast; Microbiology; Microbodies - drug effects; Microbodies - ultrastructure; Microscopy, Electron; Neurotoxins - pharmacology; Peroxisome; Peroxisomes; Pharmacology. Drug treatments; phytotoxins; Plant Proteins - pharmacology; poisonous plants; Pyrans - pharmacology; Rhamnaceae; Yeast; Yeasts; Peroxisome; Dimeric anthracenone; Methylotrophic yeast; Yeast; Anthracene derivatives; Biological activity; Fungi; Plant; Antifungal agent; Dicotyledones; Angiospermae; Plant origin; Spermatophyta; Fungi Imperfecti; Rhamnaceae; Mechanism of action; Candida boidinii; Thallophyta
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1998.tb12829.x

Dimeric anthracenones have been isolated from toxic plants of the genus Karwinskia (Rhamnaceae). T 514 or peroxisomicine A1 is one of the anthracenonic compounds which produce irreversible and selective damage on the peroxisomes of yeast cells in vivo. In this paper we describe the effect of two structurally related anthracenones on cell viability and on the peroxisomes of the methylotrophic yeast Candida boidinii. As has been described for peroxisomicine A1, peroxisomicine A2 and T 544 caused a decrease in the viability of C. boidinii at all concentrations tested, and disruption of the peroxisomal membrane, T 544 showing the strongest effect. In C. boidinii cell death and peroxisomal damage seem to be independent events.