Tissue Na+K+ activated adenosine triphosphatase in retinol deficient albino rats

The effect of retinol deficiency on Na+ K+ ATPase was studied. Retinol deficient rat kidney, liver and spleen revealed a two to three-fold increase in ouabain sensitive Na+ K+ ATPase activity in relation to controls. The activity in the controls could be stimulated more by concanavalin A than in the...

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Bibliographic Details
Published inInternational journal for vitamin and nutrition research Vol. 62; no. 4; pp. 285 - 290
Main Authors Kaul, S, Krishnakantha, T.P
Format Journal Article
LanguageEnglish
Published Switzerland 1992
Subjects
adenosinetriphosphatase
albinos
Animals
Detergents
Enzyme Activation - drug effects
Enzyme Activation - physiology
enzyme activity
Intracellular Membranes - drug effects
Intracellular Membranes - enzymology
Lectins - pharmacology
Male
Microsomes - drug effects
Microsomes - enzymology
potassium
Rats
sodium
Sodium-Potassium-Exchanging ATPase - drug effects
Sodium-Potassium-Exchanging ATPase - metabolism
Temperature
vitamin A
Vitamin A Deficiency - enzymology
vitamin deficiencies
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Summary:The effect of retinol deficiency on Na+ K+ ATPase was studied. Retinol deficient rat kidney, liver and spleen revealed a two to three-fold increase in ouabain sensitive Na+ K+ ATPase activity in relation to controls. The activity in the controls could be stimulated more by concanavalin A than in the deficient group. The membrane-bound activity in the deficient group was more susceptible to freezing and thawing than the controls. Detergents could enhance the activity in both the groups to different levels. The enzyme revealed a high Km and Vmax for ATP and the cholesterol:phospholipid ratio was markedly decreased in the deficient group.
ISSN:0300-9831