Antigens of the sheep scab mite Psoroptes ovis

Sheep scab caused by the mite Psoroptes ovis is a highly contagious disease of sheep. As a first step in developing a mite-derived vaccine for controlling the disease, the soluble antigens in mite extracts which induce an immune response in sheep were identified by electrophoretic and immunoblotting...

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Published inFolia parasitologica Vol. 45; no. 3; pp. 239 - 244
Main Authors Jayawardena, K.G.I, Heller-Haupt, A, Woodland, R.M, Varma, M.G.R. (Royal Veterinary College, Potters Bar (United Kingdom). Dept. of Farm Animal and Equine Medicine and Surgery)
Format Journal Article
LanguageEnglish
Published Czech Republic 01.01.1998
Subjects
Animals
ANTIGENS
Antigens - analysis
Antigens - chemistry
Cell Extracts
ELECTROPHORESIS
ELISA
IMMUNE RESPONSE
Immune Sera
IMMUNOBLOTTING
Mite Infestations - parasitology
Mite Infestations - veterinary
Mites - immunology
Molecular Weight
PARASITES
PSOROPTES OVIS
SHEEP
Sheep Diseases - parasitology
Species Specificity
VACCINES
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Summary:Sheep scab caused by the mite Psoroptes ovis is a highly contagious disease of sheep. As a first step in developing a mite-derived vaccine for controlling the disease, the soluble antigens in mite extracts which induce an immune response in sheep were identified by electrophoretic and immunoblotting techniques. At least 22 proteins were present in P. ovis extracts as revealed by Coomassie Blue staining. Mite-infested sheep serum recognised six antigenic bands in the extracts with approximate relative molecular weights ranging from 12 to 183 kDa. A deeply staining band at 31.2 kDa and another at 41.8 kDa are of particular diagnostic value. Immunoblotting studies showed that there was no cross reactivity between P. ovis and two other ectoparasites of sheep in the UK, the sheep louse Bovicola ovis and the sheep tick Ixodes ricinus.
Bibliography:L72
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ISSN:0015-5683
1803-6465