In vitro fertilization of bovine follicular oocytes obtained by laparoscopy
Bovine follicular oocytes (n = 454), obtained after laparoscopy, were used to study in vitro capacitation, fertilization, and embryo development. Capacitation was accomplished by treating bovine spermatozoa with high ionic strength medium. Maturation, fertilization, and development studies were carr...
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Published in | Biology of reproduction Vol. 33; no. 2; pp. 487 - 494 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Society for the Study of Reproduction
01.09.1985
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Subjects | |
Online Access | Get full text |
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Summary: | Bovine follicular oocytes (n = 454), obtained after laparoscopy, were used to study in vitro capacitation, fertilization,
and embryo development. Capacitation was accomplished by treating bovine spermatozoa with high ionic strength medium. Maturation,
fertilization, and development studies were carried out in Brackett's defined medium or in Ham's F-10. In vitro fertilization
rates, ranging from 14% to 55%, were found to be influenced by individual variations among males. Brackett's defined medium
was found to be superior to Ham's F-10 for oocyte maturation, fertilization, and growth, these media giving cleavage rates
of 60% and 32%, respectively. Oocytes with expanded cumuli at the time of recovery cleaved at a rate of 43%, which is significantly
different from oocytes recovered without granulosa cells (22%) or oocytes with compact cumuli and corona cells (5%). The in
vitro development pattern of the in vitro-fertilized embryos was found to be similar to that observed in vivo. Embryos were
observed at the 2-cell stage 44.5 +/- 6.3 h after in vitro insemination, 4-cell after 59.0 +/- 9.4 h, 8-cell after 74.8 +/-
12.7 h, and 16-cell after 96.2 +/- 13.9 h (observations at 12-h intervals). The procedures described here resulted in cleavage
rates of up to 60% using follicular oocytes embedded in expanded cumuli cells and semen samples from selected males. |
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Bibliography: | L40 8630572 L53 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod33.2.487 |